Biotransformation of 3-cyanopyridine to nicotinic acid by free and immobilized cells of recombinant Escherichia coli
- 28 January 2014
- journal article
- Published by Elsevier BV in Process Biochemistry
- Vol. 49 (4), 655-659
- https://doi.org/10.1016/j.procbio.2014.01.023
Abstract
An efficient biocatalytic process for the production of nicotinic acid (niacin) from 3-cyanopyridine was developed using cells of recombinant Escherichia coli JM109 harboring the nitrilase gene from Alcaligenes faecalis MTCC 126. The freely suspended cells of the biocatalyst were found to withstand higher concentrations of the substrate and the product without any signs of substrate inhibition. Immobilization of the cells further enhanced their substrate tolerance, stability and reusability in repetitive cycles of nicotinic acid production. Under optimized conditions (37 °C, 100 mM Tris buffer, pH 7.5) for the immobilized cells, the recombinant biocatalyst achieved a 100% conversion of 1 M 3-cyanopyridine to nicotinic acid within 5 h at a cell mass concentration (fresh weight) of 500 mg/mL. The high substrate/product tolerance and stability of the immobilized whole cell biocatalyst confers its potential industrial use.Keywords
This publication has 20 references indexed in Scilit:
- Biosynthesis of nicotinic acid from 3-cyanopyridine by a newly isolated Fusarium proliferatum ZJB-09150World Journal of Microbiology and Biotechnology, 2012
- Nicotinic acid bio-production by Microbacterium imperiale CBS 489-74: Effect of 3-cyanopyridine and temperature on amidase activityProcess Biochemistry, 2012
- Characterization of a newly isolated strain Rhodococcus erythropolis ZJB-09149 transforming 2-chloro-3-cyanopyridine to 2-chloronicotinic acidNew Biotechnology, 2011
- High-yield continuous production of nicotinic acid via nitrile hydratase–amidase cascade reactions using cascade CSMRsEnzyme and Microbial Technology, 2011
- Application of continuous stirred membrane reactor to 3-cyanopyridine bioconversion using the nitrile hydratase–amidase cascade system of Microbacterium imperiale CBS 498-74Enzyme and Microbial Technology, 2010
- Amidase-catalyzed production of nicotinic acid in batch and continuous stirred membrane reactorsEnzyme and Microbial Technology, 2008
- Nocardia globerula NHB-2: Bench scale production of nicotinic acidProcess Biochemistry, 2006
- Thermostable nitrilase catalysed production of nicotinic acid from 3-cyanopyridineEnzyme and Microbial Technology, 1999
- Influence of duodenal infusion of nicotinic acid on the milk fat composition of dairy cowsArchives of Animal Nutrition, 1997
- Conversion of 3-cyanopyridine to nicotinic acid by Nocardia rhodochrous LL100-21Enzyme and Microbial Technology, 1989