How Translocons Select Transmembrane Helices
- 1 June 2008
- journal article
- review article
- Published by Annual Reviews in Annual Review of Biophysics
- Vol. 37 (1), 23-42
- https://doi.org/10.1146/annurev.biophys.37.032807.125904
Abstract
Like all cellular proteins, membrane proteins are synthesized by ribosomes. But unlike their soluble counterparts, highly hydrophobic membrane proteins require auxiliary machineries to prevent aggregation in aqueous cellular compartments. The principal machine is the translocon, which works in concert with ribosomes to manage the orderly insertion of α-helical membrane proteins directly into the endoplasmic reticulum membrane of eukaryotes or into the plasma membrane of bacteria. In the course of insertion, membrane proteins come into thermodynamic equilibrium with the lipid membrane, where physicochemical interactions determine the final three-dimensional structure. Much progress has been made during the past several years toward understanding the physical chemistry of membrane protein stability, the structure of the translocon machine, and the mechanisms by which the translocon selects and inserts transmembrane helices. We review this progress and consider the connection between the physical principles of membrane protein stability and translocon selection of transmembrane helices.Keywords
This publication has 89 references indexed in Scilit:
- Diffraction-Based Density Restraints for Membrane and Membrane-Peptide Molecular Dynamics SimulationsBiophysical Journal, 2006
- Membrane binding of the bacterial signal recognition particle receptor involves two distinct binding sitesThe Journal of cell biology, 2006
- Disulfide bridge formation between SecY and a translocating polypeptide localizes the translocation pore to the center of SecYThe Journal of cell biology, 2005
- Experimental Validation of Molecular Dynamics Simulations of Lipid Bilayers: A New ApproachBiophysical Journal, 2005
- Computer simulations of membrane proteinsBiochimica et Biophysica Acta (BBA) - Biomembranes, 2004
- Folding Kinetics of an α Helical Membrane Protein in Phospholipid Bilayer VesiclesJournal of Molecular Biology, 2004
- The role of the ribosome-translocon complex in translation and assembly of polytopic membrane proteinsTrends in Biochemical Sciences, 1998
- Helix packing in membrane proteinsJournal of Molecular Biology, 1997
- Free Energy of Amide Hydrogen Bond Formation in Vacuum, in Water, and in Liquid Alkane SolutionThe Journal of Physical Chemistry B, 1997
- Free-energy determinants of alpha-helix insertion into lipid bilayersBiophysical Journal, 1996