Association between the endoplasmic reticulum and mitochondria of yeast facilitates interorganelle transport of phospholipids through membrane contact
Open Access
- 1 September 1999
- journal article
- research article
- Published by Wiley in JBIC Journal of Biological Inorganic Chemistry
- Vol. 264 (2), 545-553
- https://doi.org/10.1046/j.1432-1327.1999.00658.x
Abstract
Membrane association between mitochondria and the endoplasmic reticulum of the yeast Saccharomyces cerevisiae is probably a prerequisite for phospholipid translocation between these two organelles. This association was visualized by fluorescence microscopy and computer‐aided three‐dimensional reconstruction of electron micrographs from serial ultrathin sections of yeast cells. A mitochondria‐associated membrane (MAM), which is a subfraction of the endoplasmic reticulum, was isolated and re‐associated with mitochondria in vitro. In the reconstituted system, phosphatidylserine synthesized in MAM was imported into mitochondria independently of cytosolic factors, bivalent cations, ATP, and ongoing synthesis of phosphatidylserine. Proteolysis of mitochondrial surface proteins by treatment with proteinase K reduced the capacity to import phosphatidylserine. Phosphatidylethanolamine formed in mitochondria by decarboxylation of phosphatidylserine is exported to the endoplasmic reticulum where part of it is converted into phosphatidylcholine. In contrast with previous observations with permeabilized yeast cells [Achleitner, G., Zweytick, D., Trotter, P., Voelker, D. & Daum, G. (1995) J. Biol. Chem.270, 29836–29842], export of phosphatidylethanolamine from mitochondria to the endoplasmic reticulum was shown to be energy‐independent in the reconstituted yeast system.Keywords
This publication has 39 references indexed in Scilit:
- Preferential Decarboxylation of Hydrophilic Phosphatidylserine Species in Cultured CellsPublished by Elsevier BV ,1998
- Synthesis and Intracellular Transport of Aminoglycerophospholipids in Permeabilized Cells of the Yeast, Saccharomyces cerevisiaePublished by Elsevier BV ,1995
- Intracellular transport of inositol‐containing sphingolipids in the yeast, Saccharomyces cerevisiaeFEBS Letters, 1995
- Evidence That Phosphatidylserine Is Imported into Mitochondria via a Mitochondria-associated Membrane and That the Majority of Mitochondrial Phosphatidylethanolamine Is Derived from Decarboxylation of PhosphatidylserinePublished by Elsevier BV ,1995
- Characterization of a microsomal subfraction associated with mitochondria of the yeast, Saccharomyces cerevisiae. Involvement in synthesis and import of phospholipids into mitochondriaBiochimica et Biophysica Acta (BBA) - Biomembranes, 1995
- Identification of a Non-mitochondrial Phosphatidylserine Decarboxylase Activity (PSD2) in the Yeast Saccharomyces cerevisiaeJournal of Biological Chemistry, 1995
- Phospholipid trafficking and membrane biogenesisBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1994
- PHOSPHOLIPID TRANSFER PROTEINSAnnual Review of Biochemistry, 1991
- Biosynthesis of mannosylinositolphosphoceramide in Saccharomyces cerevisiae is dependent on genes controlling the flow of secretory vesicles from the endoplasmic reticulum to the Golgi.The Journal of cell biology, 1991
- Transport of cholesterol from the endoplasmic reticulum to the plasma membrane.The Journal of cell biology, 1985