Cloning of cytoplasmic heat shock protein 90 (FcHSP90) from Fenneropenaeus chinensis and its expression response to heat shock and hypoxia
- 31 July 2008
- journal article
- research article
- Published by Springer Science and Business Media LLC in Cell Stress and Chaperones
- Vol. 14 (2), 161-172
- https://doi.org/10.1007/s12192-008-0069-6
Abstract
Heat shock protein 90 (HSP90) works as a multi-functional chaperone and is involved in the regulation of many essential cellular pathways. In this study, we have identified a full-length complementary DNA (cDNA) of HSP90 (FcHSP90) from Chinese shrimp Fenneropenaeus chinensis. FcHSP90 full-length cDNA comprised 2,552 bp, including a 2,181-bp open reading frame encoding 726 amino acids. Both homology analyses using alignment with previously identified HSP90 and a phylogeny tree indicated that FcHSP90 was a cytoplasmic HSP90. Real-time reverse transcription polymerase chain reaction analysis revealed that FcHSP90 was ubiquitously expressed in all the examined tissues but with highest levels in ovary of F. chinensis. FcHSP90 mRNA levels were sensitively induced by heat shock (from 25°C to 35°C) and reached the maximum at 6 h during heat shock treatment. Under hypoxia conditions, FcHSP90 mRNA levels, in both hemocytes and gill, were induced at 2 h and depressed at 8 h during hypoxia stress. The assessment of FcHSP90 mRNA levels under heat shock and hypoxia stresses indicated that the transcription of FcHSP90 was very sensitive to heat shock and hypoxia, so we deduced that FcHSP90 might play very important roles for shrimp to cope with environmental stress.Keywords
This publication has 53 references indexed in Scilit:
- COMPASS: A suite of pre‐ and post‐search proteomics software tools for OMSSAProteomics, 2011
- Hsp90—From signal transduction to cell transformationBiochemical and Biophysical Research Communications, 2007
- Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditionsThe FEBS Journal, 2005
- The role of Hsp90 in cell response to hyperthermiaJournal of Thermal Biology, 2004
- Aryl Hydrocarbon Nuclear Translocator (ARNT) Promotes Oxygen-independent Stabilization of Hypoxia-inducible Factor-1α by Modulating an Hsp90-dependent Regulatory PathwayOnline Journal of Public Health Informatics, 2004
- Cloning, sequencing and expression analysis of cDNA encoding a constitutive heat shock protein 70 (HSC70) in Fenneropenaeus chinensisChinese Science Bulletin, 2004
- Can the (M• — X) Region in Electron Capture Dissociation Provide Reliable Information on Amino Acid Composition of Polypeptides?European Journal of Mass Spectrometry, 2002
- Molecular Chaperones in the Cytosol: from Nascent Chain to Folded ProteinScience, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Expression of heat-shock locus hsr-omega in nonstressed cells during development in Drosophila melanogasterDevelopmental Biology, 1991