Age, Genetic Characteristics and Number of Cycles Are Critical Factors to Consider for Successful Protection of the Murine Heart With Postconditioning
- 16 December 2011
- journal article
- Published by Institute of Physiology of the Czech Academy of Sciences in Physiological Research
- Vol. 60 (6), 971-974
- https://doi.org/10.33549/physiolres.932129
Abstract
Postconditioning (PostC) is a recently discovered phenomenon whereby brief repetitive cycles of ischaemia with intermittent reperfusion following prolonged ischaemia elicit cardioprotection. This study investigated whether the age, genetic characteristics or number of repetitive cycles influenced the protective effect of PostC in mice. C57BL/6 floxed or non-floxed STAT-3 mice aged between 14-16 weeks (young) or 18-20 weeks (older) were perfused on a Langendorff apparatus and subjected to 35 min global ischaemia and 45 min reperfusion. PostC was elicited by either 3 (PostC-3) or 6 cycles (PostC-6) of 10s ischaemia and 10 s reperfusion. PostC-3 and PostC-6 in both young and older non-floxed mice reduced the myocardial infarct size. In contrast, only PostC-3 reduced myocardial infarct size in young floxed mice. Neither PostC-3 nor PostC-6 reduced the infarct in older floxed mice. Our data reveal that genetic characteristics, a minute difference in age or the number of postconditioning cycles are critical factors to be considered for the successful effect of ischaemic postconditioning in a murine model. Moreover, these factors should be taken into consideration for future experimental research or clinical applications of this protective phenomenon.Keywords
This publication has 13 references indexed in Scilit:
- Continuous Glycoprotein-130–Mediated Signal Transducer and Activator of Transcription-3 Activation Promotes Inflammation, Left Ventricular Rupture, and Adverse Outcome in Subacute Myocardial InfarctionCirculation, 2010
- Ischaemic postconditioning protects against reperfusion injury via the SAFE pathwayCardiovascular Research, 2009
- Ischemic Postconditioning in PigsCirculation Research, 2009
- Glycogen Synthase Kinase-3 Inactivation Is Not Required for Ischemic Preconditioning or Postconditioning in the MouseCirculation Research, 2008
- A floxed allele of the androgen receptor gene causes hyperandrogenization in male micePhysiological Genomics, 2008
- Cardioprotection by Ischemic Postconditioning Is Lost in Aged and STAT3-Deficient MiceCirculation Research, 2008
- Ischemic postconditioning during reperfusion activates Akt and ERK without protecting against lethal myocardial ischemia-reperfusion injury in pigsAmerican Journal of Physiology-Heart and Circulatory Physiology, 2006
- Postconditioning in rats and miceScandinavian Cardiovascular Journal, 2006
- Genetic depletion of cardiac myocyte STAT-3 abolishes classical preconditioningCardiovascular Research, 2004
- Signal Transducer and Activator of Transcription 3 Is Required for Myocardial Capillary Growth, Control of Interstitial Matrix Deposition, and Heart Protection From Ischemic InjuryCirculation Research, 2004