Common antigenicity between Japanese cedar (Cryptomeria japonica) pollen and Japanese cypress (Chamaecyparis obtusa) pollen, II. Determination of the cross‐reacting T‐cell epitope of Cry j 1 and Cha o 1 in mice
Open Access
- 1 April 2000
- journal article
- Published by Wiley in Immunology
- Vol. 99 (4), 630-634
- https://doi.org/10.1046/j.1365-2567.2000.00021.x
Abstract
We have previously detected common antigenicity between Cry j 1 and Cha o 1 in B10.S mice. B10.S mice immunized with Cry j 1‐ or Cha o 1‐generated T cells and antibodies reactive to both allergens. In the present study, we investigated the cross‐reacting and Cry j 1‐specific T‐cell epitopes in B10.S mice. Lymph node cells from B10.S mice immunized with Cry j 1 recognized Cry j 1 p111–130, p211–230, and p310–330 as well as Cha o 1 p209–228. The existence of the cross‐reacting T‐cell epitope in Cry j 1 and Cha o 1 was confirmed by the response of newly established p211–230‐specific and Cha o 1 p209–228‐specific T‐cell lines. The minimum peptide sequence (p213–224) of the cross‐reacting T‐cell epitope was identical in Cry j 1 and Cha o 1. These findings clearly demonstrate that common antigenicity at the T‐cell level between Japanese cedar and cypress pollen allergens was caused by the existence of an identitical‐cell epitope in Cry j 1 and Cha o 1.Keywords
This publication has 13 references indexed in Scilit:
- Common antigenicity between Japanese cedar (Cryptomeria japonica ) pollen and Japanese cypress (Chamaecyparis obtusa ) pollen, I. H-2 complex affects cross responsiveness to Cry j 1 and Cha o 1 at the T- and B-cell level in miceImmunology, 2000
- Termination of Peripheral Tolerance to a T Cell Epitope by Heteroclitic Antigen AnaloguesThe Journal of Immunology, 1998
- T Cell Epitopes in Japanese Cedar (Cryptomeria japonica) Pollen Allergens: Choice of Major T Cell Epitopes in Cry j 1 and Cry j 2 Toward Design of the Peptide-Based Immunotherapeutics for the Management of Japanese Cedar PollinosisThe Journal of Immunology, 1998
- The peptide-binding strategy of the MHC class II I-A moleculesImmunology Today, 1998
- Clinical effects of immunotherapy on Japanese cedar pollinosis in the season of cedar and cypress pollinationAuris Nasus Larynx, 1997
- Human T Cell Response to Antigen Peptides of Japanese Cedar Pollen (Cry j 1)International Archives of Allergy and Immunology, 1997
- Purification, characterization and molecular cloning of Cha o 1, a major allergen of Chamaecyparis obtusa (Japanese cypress) pollenMolecular Immunology, 1996
- Specific IgE to Japanese cypress (Chamaecyparis obtusa) in patients with nasal allergy.1995
- Detection of Specific IgE Antibodies to Japanese Cypress Pollen in Patients with Nasal Allergy: A Comparative Study with Japanese CedarAuris Nasus Larynx, 1995
- Epitopes on Cry j I and Cry j II for the human ige antibodies cross-reactive between Cupressus sempervirens and Cryptomeria japonica pollenMolecular Immunology, 1993