Arabidopsis boron transporter for xylem loading
- 1 November 2002
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 420 (6913), 337-340
- https://doi.org/10.1038/nature01139
Abstract
Boron deficiency hampers the productivity of 132 crops in more than 80 countries. Boron is essential in higher plants primarily for maintaining the integrity of cell walls and is also beneficial and might be essential in animals and in yeast. Understanding the molecular mechanism(s) of boron transport is crucial for alleviating boron deficiency. Here we describe the molecular identification of boron transporters in biological systems. The Arabidopsis thaliana mutant bor1-1 is sensitive to boron deficiency. Uptake studies indicated that xylem loading is the key step for boron accumulation in shoots with a low external boron supply and that the bor1-1 mutant is defective in this process. Positional cloning identified BOR1 as a membrane protein with homology to bicarbonate transporters in animals. Moreover, a fusion protein of BOR1 and green fluorescent protein (GFP) localized to the plasma membrane in transformed cells. The promoter of BOR1 drove GFP expression in root pericycle cells. When expressed in yeast, BOR1 decreased boron concentrations in cells. We show here that BOR1 is an efflux-type boron transporter for xylem loading and is essential for protecting shoots from boron deficiency.Keywords
This publication has 27 references indexed in Scilit:
- Update on Boron in Higher Plants ‐ Uptake, Primary Translocation and CompartmentationPlant Biology, 2002
- Boron in Plant BiologyPlant Biology, 2002
- Requirement of Borate Cross-Linking of Cell Wall Rhamnogalacturonan II for Arabidopsis GrowthScience, 2001
- Preferential translocation of boron to young leaves inArabidopsis thalianaRegulated by theBOR1GeneSoil Science and Plant Nutrition, 2001
- Permeability and Channel-Mediated Transport of Boric Acid across Membrane Vesicles Isolated from Squash RootsPlant Physiology, 2000
- Defect in Root-Shoot Translocation of Boron in Arabidopsis thaliana Mutant bor 1-1Journal of Plant Physiology, 2000
- Engineered GFP as a vital reporter in plantsCurrent Biology, 1996
- Effects of Sulfur Nutrition on Expression of the Soybean Seed Storage Protein Genes in Transgenic PetuniaPlant Physiology, 1992
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- SHORT‐ AND LONG‐DISTANCE TRANSPORT OF BORIC ACID IN PLANTSNew Phytologist, 1980