Comparison of Gull Feces-Specific Assays Targeting the 16S rRNA Genes of Catellicoccus marimammalium and Streptococcus spp
Open Access
- 15 March 2012
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 78 (6), 1909-1916
- https://doi.org/10.1128/aem.07192-11
Abstract
Two novel gull-specific quantitative PCR (qPCR) assays were developed using 16S rRNA gene sequences from gull fecal clone libraries: a SYBR green assay targeting Streptococcus spp. (gull3) and a hydrolysis TaqMan assay targeting Catellicoccus marimammalium (gull4). The objectives of this study were to compare the host specificity of a previous C. marimammalium qPCR assay (gull2) with that of the new markers and to examine the presence of the three gull markers in environmental water samples from different geographic locations. Most of the gull fecal samples tested ( n = 255) generated positive signals with the gull2 and gull4 assays (i.e., >86%), whereas only 28% were positive with gull3. Low prevalence and abundance of tested gull markers (0.6 to 15%) were observed in fecal samples from six nonavian species ( n = 180 fecal samples), whereas the assays cross-reacted to some extent (13 to 31%) with other (nongull) avian fecal samples. The gull3 assay was positive against fecal samples from 11 of 15 avian species, including gull. Of the presumed gull-impacted water samples ( n = 349), 86%, 59%, and 91% were positive with the gull2, the gull3, and the gull4 assays, respectively. Approximately 5% of 239 non-gull-impacted water samples were positive with the gull2 and the gull4 assays, whereas 21% were positive witg the gull3 assay. While the relatively high occurrence of gull2 and gull4 markers in waters impacted by gull feces suggests that these assays could be used in environmental monitoring studies, the data also suggest that multiple avian-specific assays will be needed to accurately assess the contribution of different avian sources in recreational waters.Keywords
This publication has 39 references indexed in Scilit:
- Molecular Detection of Campylobacter spp. in California Gull (Larus californicus) ExcretaApplied and Environmental Microbiology, 2011
- Traditional and molecular analyses for fecal indicator bacteria in non-point source subtropical recreational marine watersWater Research, 2010
- Evaluation of Swine-Specific PCR Assays Used for Fecal Source Tracking and Analysis of Molecular Diversity of Swine-Specific “ Bacteroidales ” PopulationsApplied and Environmental Microbiology, 2009
- Bacteroidales Diversity in Ring-Billed Gulls ( Laurus delawarensis ) Residing at Lake Michigan BeachesApplied and Environmental Microbiology, 2009
- The Influenza Virus EnigmaCell, 2009
- The Ribosomal Database Project: improved alignments and new tools for rRNA analysisNucleic Acids Research, 2008
- Phylogenetic Diversity and Molecular Detection of Bacteria in Gull FecesApplied and Environmental Microbiology, 2008
- Performance, Design, and Analysis in Microbial Source Tracking StudiesApplied and Environmental Microbiology, 2007
- NAST: a multiple sequence alignment server for comparative analysis of 16S rRNA genesNucleic Acids Research, 2006
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997