Microfluidic Platforms for Single-Cell Analysis
- 1 July 2010
- journal article
- review article
- Published by Annual Reviews in Annual Review of Biomedical Engineering
- Vol. 12 (1), 187-201
- https://doi.org/10.1146/annurev-bioeng-070909-105238
Abstract
Microfluidics, the study and control of the fluidic behavior in microstructures, has emerged as an important enabling tool for single-cell chemical analysis. The complex procedures for chemical cytometry experiments can be integrated into a single microfabricated device. The capability of handling a volume of liquid as small as picoliters can be utilized to manipulate cells, perform controlled cell lysis and chemical reactions, and efficiently minimize sample dilution after lysis. The separation modalities such as chromatography and electrophoresis within microchannels are incorporated to analyze various types of intracellular components quantitatively. The microfluidic approach offers a rapid, accurate, and cost-effective tool for single-cell biology. We present an overview of the recent developments in microfluidic technology for chemical-content analysis of individual cells.Keywords
This publication has 92 references indexed in Scilit:
- Droplet microfluidic technology for single-cell high-throughput screeningProceedings of the National Academy of Sciences of the United States of America, 2009
- Assembling the Marine Metagenome, One Cell at a TimePLOS ONE, 2009
- Microfluidic single-cell analysis of intracellular compoundsJournal of The Royal Society Interface, 2008
- 96-Well Polycarbonate-Based Microfluidic Titer Plate for High-Throughput Purification of DNA and RNAAnalytical Chemistry, 2008
- Examination of laser microbeam cell lysis in a PDMS microfluidic channel using time-resolved imagingLab on a Chip, 2008
- Intuitive, Image-Based Cell Sorting Using Optofluidic Cell SortingAnalytical Chemistry, 2007
- Nanoliter Reactors Improve Multiple Displacement Amplification of Genomes from Single CellsPLoS Genetics, 2007
- Dissecting biological “dark matter” with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouthProceedings of the National Academy of Sciences of the United States of America, 2007
- Stochastic mRNA Synthesis in Mammalian CellsPLoS Biology, 2006
- Systems Biology and New Technologies Enable Predictive and Preventative MedicineScience, 2004