Antitumor efficacy induced by a B16F10 tumor cell vaccine treated with mitoxantrone alone or in combination with reserpine and verapamil in mice
Open Access
- 14 June 2011
- journal article
- Published by Spandidos Publications in Experimental and Therapeutic Medicine
- Vol. 2 (5), 911-916
- https://doi.org/10.3892/etm.2011.283
Abstract
An apoptotic tumor cell serves as a potential potent trigger for the initiation of naturally occurring tumor immunity. In the present study, a B16F10 tumor cell vaccine treated with mitoxantrone (MIT) was developed, and its antitumor effect on mice was evaluated. The results indicated that the B16F10 tumor cell vaccine treated with MIT alone or in combination with reserpine (RP) and verapamil (VP) for 12 h triggered apoptosis, and that the expression of CD80, the MHC II class molecule, NKG2D and its ligand were significantly increased compared to the expression levels in the control group. The tumor vaccine immunogenicity was significantly enhanced in the vaccinated mice, resulting in augmented cytotoxicity of splenocytes and NK cells as well as the splenocyte proliferative response compared to the control group mice. Notably, the mice vaccinated with the B16F10 tumor cell vaccine treated with MIT, RP and VP did not generate tumors only after 60 days into the observation, but the mice also generated a powerful immune prophylactic efficiency against the B16F10 tumor cell challenge. These findings demonstrated the safety and efficacy of the B16F10 tumor cell vaccine treated with MIT alone or in combination with RP and VP in the murine model, and suggest that an apoptotic tumor cell vaccine modeled on naturally occurring tumor immune responses in vivo may provide a safe and immunogenic tumor vaccine for potential applications in humans.Keywords
This publication has 27 references indexed in Scilit:
- Roles of the Ribosomal Protein S19 Dimer and Chemically Induced Apoptotic Cells as a Tumor Vaccine in Syngeneic Mouse Transplantation ModelsJournal of Immunotherapy, 2011
- Harnessing Naturally Occurring Tumor Immunity: A Clinical Vaccine Trial in Prostate CancerPLOS ONE, 2010
- Investigation on the anti-tumor efficacy by expression of GPI-anchored mIL-21 on the surface of B16F10 cells in C57BL/6 miceImmunobiology, 2010
- Mitoxantrone-Mediated Apoptotic B16-F1 Cells Induce Specific Anti-tumor Immune ResponseCellular & Molecular Immunology, 2009
- Antitumor efficacy induced by human ovarian cancer cells secreting IL-21 alone or combination with GM-CSF cytokines in nude mice modelImmunobiology, 2009
- Comparison of Immune Responses Induced in Mice by Vaccination with DNA Vaccine Constructs Expressing Mycobacterial Antigen 85A and Interleukin-21 and Bacillus Galmette-GuérinImmunological Investigations, 2008
- Recent advances in cancer stem/progenitor cell research: therapeutic implications for overcoming resistance to the most aggressive cancersJournal of Cellular and Molecular Medicine, 2007
- Cell-Surface Calreticulin Initiates Clearance of Viable or Apoptotic Cells through trans-Activation of LRP on the PhagocyteCell, 2005
- Recognition of Stress-Induced MHC Molecules by Intestinal Epithelial γδ T CellsScience, 1998
- Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo.The Journal of Experimental Medicine, 1996