A new human leukemia cell 8.2 kDa differentiation factor: Isolation and primary structure
- 19 December 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 356 (2-3), 327-329
- https://doi.org/10.1016/0014-5793(94)01306-3
Abstract
A new 8.2 kDa differentiation factor has been purified to homogeneity from the cultural media of human myelogenous HL-60 leukemia cells induced by retinoic acid. cDNA clones encoding this factor were isolated from a cDNA library prepared from HL-60 differentiated cells and their nucleotide sequence has been determined. The deduced amino acid sequence of the differentiation factor molecule consists of 54 amino acid residues. The protein is shown to be glycosylated. It was shown by Northern blot experiments that the level of poly(A)+ RNA with a length of 450 nucleotides was higher in differentiated cells than in non-differentiated cellsThis publication has 9 references indexed in Scilit:
- Eukaryotic start and stop translation sitesNucleic Acids Research, 1991
- Retinoic acid‐induced differentiation of the human promyelocytic leukemia cell line, HL‐60, and fresh human leukemia cells in primary culture: A model for differentiation inducing therapy of leukemiaEuropean Journal of Haematology, 1987
- Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extractionAnalytical Biochemistry, 1987
- Autocrine β-related interferon controls c-myc suppression and growth arrest during hematopoietic cell differentiationCell, 1986
- Induction of β2-interferon by tumor necrosis factor: A homeostatic mechanism in the control of cell proliferationCell, 1986
- Retinoic acid. Inhibition of the clonal growth of human myeloid leukemia cells.JCI Insight, 1982
- Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid.Proceedings of the National Academy of Sciences of the United States of America, 1980
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences of the United States of America, 1977
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970