Calbindin and parvalbumin are early markers of non‐mitotically regenerating hair cells in the bullfrog vestibular otolith organs
- 1 July 1997
- journal article
- Published by Wiley in International Journal of Developmental Neuroscience
- Vol. 15 (4-5), 417-432
- https://doi.org/10.1016/s0736-5748(96)00101-3
Abstract
Earlier studies have demonstrated hair cell regeneration in the absence of cell proliferation, and suggested that supporting cells could phenotypically convert into hair cells following hair cell loss. Because calcium-binding proteins are involved in gene up-regulation, cell growth, and cell differentiation, we wished to determine if these proteins were up-regulated in scar formations and regenerating hair cells following gentamicin treatment. Calbindin and parvalbumin immunolabeling was examined in control or gentamicin-treated (GT) bullfrog saccular and utricular explants cultured for 3 days in amphibian culture medium or amphibian culture medium supplemented with aphidicolin, a blocker of nuclear DNA replication in eukaryotic cells. In control cultures, calbindin and parvalbumin immunolabeled the hair bundles and, less intensely, the cell bodies of mature hair cells. In GT or mitotically-blocked GT (MBGT) cultures, calbindin and parvalbumin immunolabeling was also seen in the hair bundles, cuticular plates, and cell bodies of hair cells with immature hair bundles. Thus, these antigens were useful markers for both normal and regenerating hair cells. Supporting cell immunolabeling was not seen in control cultures nor in the majority of supporting cells in GT cultures. In MBGT cultures, calbindin and parvalbumin immunolabeling was up-regulated in the cytosol of single supporting cells participating in scar formations and in supporting cells with hair cell-like characteristics. These data provide further evidence that non-mitotic hair cell regeneration in cultures can be accomplished by the conversion of supporting cells into hair cells.Keywords
This publication has 61 references indexed in Scilit:
- Intracellular distributions and putative functions of calcium-binding proteins in the bullfrog vestibular otolith organsHearing Research, 1997
- Calcium-binding proteins in the inner ear ofXenopus laevis (Daudin)Brain Research, 1993
- Changes in Ca2+-binding proteins in human neurodegenerative disordersTrends in Neurosciences, 1992
- L-type voltage-sensitive calcium channels mediate synaptic activation of immediate early genesNeuron, 1991
- High-purity isolation of bullfrog hair bundles and subcellular and topological localization of constituent proteins.The Journal of cell biology, 1991
- Calbindin (CaBP 28 kDa) appearance and distribution during development of the mouse inner earDevelopmental Brain Research, 1988
- Actin filaments, stereocilia, and hair cells of the bird cochleaDevelopmental Biology, 1986
- Voltage- and ion-dependent conductances in solitary vertebrate hair cellsNature, 1983
- Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.Journal of Histochemistry & Cytochemistry, 1981
- Intercellular junctions in the reticular lamina of the organ of CortiJournal of Neurocytology, 1976