Architectural limits on split genes
- 24 December 1996
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 93 (26), 15081-15085
- https://doi.org/10.1073/pnas.93.26.15081
Abstract
Exon/intron architecture varies across the eukaryotic kingdom with large introns and small exons the rule in vertebrates and the opposite in lower eukaryotes. To investigate the relationship between exon and intron size in pre-mRNA processing, internally expanded exons were placed in vertebrate genes with small and large introns. Both exon and intron size influenced splicing phenotype. Intron size dictated if large exons were efficiently recognized. When introns were large, large exons were skipped; when introns were small, the same large exons were included. Thus, large exons were incompatible for splicing if and only if they were flanked by large introns. Both intron and exon size became problematic at approximately 500 nt, although both exon and intron sequence influenced the size at which exons and introns failed to be recognized. These results indicate that present-day gene architecture reflects at least in part limitations on exon recognition. Furthermore, these results strengthen models that invoke pairing of splice sites during recognition of pre-mRNAs, and suggest that vertebrate consensus sequences support pairing across either introns or exons.Keywords
This publication has 24 references indexed in Scilit:
- Initial splice-site recognition and pairing during pre-mRNA splicingCurrent Opinion in Genetics & Development, 1996
- Exon Recognition in Vertebrate SplicingJournal of Biological Chemistry, 1995
- Two different sequence elements within exon 4 are necessary for calcitonin-specific splicing of the human calcitonin/calcitonin gene-related peptide I pre-mRNA.Molecular and Cellular Biology, 1994
- A splicing enhancer complex controls alternative splicing of doublesex pre-mRNACell, 1993
- Alternative splicing in the neural cell adhesion molecule pre-mRNA: regulation of exon 18 skipping depends on the 5'-splice site.Genes & Development, 1991
- Messenger RNA Splicing in Yeast: Clues to Why the Spliceosome Is a RibonucleoproteinScience, 1991
- Pre-mRNA splicing in yeastTrends in Genetics, 1991
- Does steric interference between splice sites block the splicing of a short c-src neuron-specific exon in non-neuronal cells?Genes & Development, 1991
- Exon definition may facilitate splice site selection in RNAs with multiple exons.Molecular and Cellular Biology, 1990
- A survey on intron and exon lengthsNucleic Acids Research, 1988