Ca2+sparks and T tubule reorganization in dedifferentiating adult mouse skeletal muscle fibers

Abstract

Ca⁺sparks are rare in healthy adult mammalian skeletal muscle but may appear when adult fiber integrity is compromised, and occur in embryonic muscle but decline as the animal develops. Here we used cultured adult mouse flexor digitorum brevis muscle fibers to monitor occurrence of Ca²⁺sparks during maintenance of adult fiber morphology and during eventual fiber morphological dedifferentiation after various times in culture. Fibers cultured for up to 3 days retain normal morphology and striated appearance. Ca²⁺sparks were rare in these fibers. At 5–7 days in culture, many of the original muscle fibers exhibit sprouting and loss of striations, as well as the occurrence of spontaneous Ca²⁺sparks. The average rate of occurrence of Ca²⁺sparks is >10-fold higher after 5–7 days in culture than in days 1–3. With the use of fibers cultured for 7 days, application of the Ca²⁺channel blockers Co²⁺or nifedipine almost completely suppressed the occurrence of Ca²⁺sparks, as previously shown in embryonic fibers, suggesting that Ca²⁺sparks may be generated by similar mechanisms in dedifferentiating cultured adult fibers and in embryonic fibers before final differentiation. The sarcomeric disruption observed under transmitted light microscopy in dedifferentiating fibers was accompanied by morphological changes in the transverse (T) tubular system, as observed by fluorescence confocal imaging of both an extracellular marker dye and membrane staining dyes. Changes in T tubule morphology coincided with the appearance of Ca²⁺sparks, suggesting that Ca²⁺sparks may either be a signal for, or the result of, disruption of DHPR-ryanodine receptor 1 coupling.