Basic Techniques in Mammalian Cell Tissue Culture
Open Access
- 2 March 2015
- journal article
- unit
- Published by Wiley in Current Protocols in Cell Biology
- Vol. 66 (1), 1.1.1-1.1.22
- https://doi.org/10.1002/0471143030.cb0101s66
Abstract
Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. © 2015 by John Wiley & Sons, Inc.Keywords
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