Presence of spectrin in untreated friend erythroleukemic cells. Its accumulation upon treatment of the cells with dimethyl sulfoxide
- 1 December 1978
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 97 (3), 293-304
- https://doi.org/10.1002/jcp.1040970304
Abstract
Friend leukemia cells (FLC) are nucleated erythroid precursors, and are markedly stimulated towards more advanced stages of differentiation by treatment with dimethyl sulfoxide (DMSO). The presence of spectrin, an erythrocyte membrane protein, has been investigated in untreated and in DMSO‐treated FLC by indirect immunofluorescence and by analysis in SDS‐polyacrylamide gel electrophoresis of low‐ionic‐strength cell extracts immuno‐precipitated with a monospecific anti‐spectrin serum. Spectrin is detectable in significant amounts in the “inducible” clones prior to DMSO stimulation, and accumulates 4‐ to 5‐fold upon addition of this compound to the cultures. Spectrin accumulation occurs rather early (24 hours after cell seeding) and reaches its peak on the third day, to decline thereafter. Semiquantitative determinations of spectrin amounts present in DMSO‐stimulated 745A and A°1 cells on the third day after treatment were 2.4 × 105 and 3.0 × 105 molecules/cell, respectively. Spectrin is also detectable in very low amounts in an “uninducible” line of FLC, and is not accumulated upon DMSO treatment thereof, whereas treatment with hemin does cause a significant increase of spectrin‐positive cells. These data indicate that spectrin is a convenient “early” marker for in vitro studies of erythropoiesis.This publication has 22 references indexed in Scilit:
- On the mechanism of ATP-induced shape changes in the human erythrocyte membranes: the role of ATPThe Journal of cell biology, 1977
- On the mechanism of ATP-induced shape changes in human erythrocyte membranes. I. The role of the spectrin complex.The Journal of cell biology, 1977
- Spectrin is absent in various tissue culture cellsNature, 1977
- Relations of the spectrin complex of human erythrocyte membranes to the actomyosins of muscle cellBiochemistry, 1976
- Purification of globin messenger RNA from dimethylsulfoxide-induced friend cells and detection of a putative globin messenger RNA precursorJournal of Molecular Biology, 1976
- Induction of globin mRNA accumulation by hemin in cultured erythroleukemic cellsCell, 1976
- Erythrocyte spectrin. Purification in deoxycholate and preliminary characterizationBiochemistry, 1976
- Transmembrane control of the receptors on normal and tumor cellsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1976
- The dynamic state of the lymphocyte membrane. Factors affecting the distribution and turnover of surface immunoglobulinsEuropean Journal of Immunology, 1972
- Une micro-méthode de l’immuno-électrophorèseInternational Archives of Allergy and Immunology, 1955