FC-RECEPTORS INDUCED BY HERPES-SIMPLEX VIRUS .1. BIOLOGIC AND BIOCHEMICAL PROPERTIES

Abstract

Radioiodinated normal rabbit Ig[immunoglobulin]G was used to detect Fc receptors on the surface of HeLa S3-2 [human cervical carcinoma] cells infected with herpes simplex virus (HSV). Unlike the Fc receptors present on most leukocytes, these virus-induced Fc receptors were sensitive to trypsin at concentrations of enzyme as low as 0.1 mg/ml. Treatment of infected cells with neuraminidase enhanced the binding of IgG. The HSV-induced Fc receptor(s) is probably a glycoprotein because its synthesis was inhibited by 2-deoxy-D-glucose at concentrations inhibiting glycoproteins but not total proteins. Binding of radioiodinated IgG to Fc receptors on infected HeLa S3-2 cells was unaffected by F(ab'')2 fragments prepared from antisera against uninfected HeLa S3-2 cells or against human .beta.2-microglobulin. Anti-HSV F(ab'')2 or Fab'' fragments decreased binding of radioiodinated IgG to HSV-infected cells by 85%, and binding of radioiodinated Fc was also inhibited by anti-HSV Fab''.