Effects of Nebivolol on Endothelial Gene Expression during Oxidative Stress in Human Umbilical Vein Endothelial Cells
Open Access
- 17 April 2008
- journal article
- research article
- Published by Hindawi Limited in Mediators of Inflammation
- Vol. 2008, 1-6
- https://doi.org/10.1155/2008/367590
Abstract
The endothelium plays a key role in the development of atherogenesis and its inflammatory and proliferative status influences the progression of atherosclerosis. The aim of this study is to compare the effects of two beta blockers such as nebivolol and atenolol on gene expression in human umbilical vein endothelial cells (HUVECs) following an oxidant stimulus. HUVECs were incubated with nebivolol or atenolol (10 micromol/L) for 24 hours and oxidative stress was induced by the addition of oxidized (ox)-LDL. Ox-LDL upregulated adhesion molecules (ICAM-1, ICAM-2, ICAM-3, E-selectin, and P-selectin); proteins linked to inflammation (IL-6 and TNFalpha), thrombotic state (tissue factor, PAI-1 and uPA), hypertension such as endothelin-1 (ET-1), and vascular remodeling such as metalloproteinases (MMP-2, MMP-9) and protease inhibitor (TIMP-1). The exposure of HUVECs to nebivolol, but not to atenolol, reduced these genes upregulated by oxidative stress both in terms of protein and RNA expression. The known antioxidant properties of the third generation beta blocker nebivolol seem to account to the observed differences seen when compared to atenolol and support the specific potential protective role of this beta blocker on the expression of a number of genes involved in the initiation and progression of atherosclerosis.Keywords
This publication has 42 references indexed in Scilit:
- Carvedilol, a pharmacological antioxidant, inhibits neointimal matrix metalloproteinase-2 and -9 in experimental atherosclerosisFree Radical Biology & Medicine, 2007
- Effect of dl-nebivolol, its enantiomers and metabolites on the intracellular production of superoxide and nitric oxide in human endothelial cellsPharmacological Research, 2007
- Influence of Nebivolol and Metoprolol on Inflammatory Mediators in Human Coronary Endothelial or Smooth Muscle Cells. Effects on Neointima Formation After Balloon Denudation in Carotid Arteries of Rats Treated with NebivololCellular Physiology and Biochemistry, 2007
- Nebivolol Inhibits Superoxide Formation by NADPH Oxidase and Endothelial Dysfunction in Angiotensin II–Treated RatsHypertension, 2006
- Effects of oxidized low‐ and high‐density lipoproteins on gene expression of human macrophagesScandinavian Journal of Clinical and Laboratory Investigation, 2006
- Protein chip-based microarray profiling of oxidized low density lipoprotein-treated cellsProteomics, 2005
- Circulating adhesion molecules levels in type 2 diabetes mellitus and hypertensionInternational Journal of Cardiology, 2005
- Inflammation in atherosclerosisNature, 2002
- β-Blockers of the Third Generation Inhibit Endothelin-1 Liberation, mRNA Production and Proliferation of Human Coronary Smooth Muscle and Endothelial CellsJournal of Cardiovascular Pharmacology, 2000
- Absence of P-selectin delays fatty streak formation in mice.JCI Insight, 1997