A haplotype containing thep53polymorphisms Ins16bp and Arg72Pro modifies cancer risk inBRCA2mutation carriers
- 17 January 2006
- journal article
- research article
- Published by Hindawi Limited in Human Mutation
- Vol. 27 (3), 242-248
- https://doi.org/10.1002/humu.20283
Abstract
Germline mutations in the BRCA1 and BRCA2 genes confer a high lifetime risk of developing breast and other cancers; however, remarkable differences exist regarding disease manifestation in mutation carriers. It has been suggested that other genetic and/or environmental factors modify not only the appearance but also the age of onset and type of tumor in BRCA1/2‐associated cases. The aim of the present study was to investigate the role of two p53 polymorphisms (c.97‐147ins16bp and c.215c>g, p.Arg72Pro) as potential modifiers. For this purpose we investigated the possible association between the two polymorphisms and disease status in 447 BRCA1/2 mutation carriers belonging to 170 Spanish breast and/or ovarian cancer families. Genotype and haplotype analyses revealed that the presence of a specific haplotype carrying the allele without the 16‐bp insertion and the variant allele for the Arg72Pro (No Ins‐72Pro haplotype) was associated with an earlier age of onset in BRCA2 mutation carriers. We found an increased risk of developing a first primary tumor (breast or ovarian) before 35 years of age for individuals who carried at least one No Ins‐72Pro haplotype (OR: 2.69; 95% CI: 1.15–6.29; P=0.022). We confirmed these data by a functional study in which we compared different p53 genotypes in relation to their apoptotic response after cell treatment with a cytotoxic drug (AraC). Our results revealed a decrease in p53 apoptotic rate associated with the No Ins‐72Pro haplotype. Hum Mutat 27(3), 242–248, 2006.Keywords
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