Ginsenoside Rh2 inhibits proliferation and induces apoptosis in human leukemia cells via TNF-α signaling pathway
- 1 August 2016
- journal article
- research article
- Published by China Science Publishing & Media Ltd. in Acta Biochimica et Biophysica Sinica
- Vol. 48 (8), 750-755
- https://doi.org/10.1093/abbs/gmw049
Abstract
Ginsenoside Rh2, a triterpene saponin extracted from Panax ginseng, exhibits pharmacological activity against multiple cancers. However, the anticancer mechanism of ginsenoside Rh2 is unclear. In this study, we found that ginsenoside Rh2 effectively inhibits growth and induces apoptosis of HL-60 cells. Using microarray technology, we found that tumor necrosis factor-α (TNF-α) is clearly up-regulated. Furthermore, anti-TNF-α antibody relieved the Rh2-induced HL-60 cell apoptosis via suppression of caspase-8, caspase-9, and caspase-3 activation. In addition, TNF-α up-regulation was also observed in other Rh2-treated cancer cell lines. These results demonstrate that TNF-α plays a key role in ginsenoside Rh2-induced cell apoptosis.Keywords
Funding Information
- National Natural Science Foundation of China (81071821, 30572208, and 30470189)
- Key Hunan Provincial Natural Science Foundation of China (13JJ2016 and 07JJ5096)
- Science and Technology Program of Hunan Province of China (2014SK4050)
- Changsha National High Tech Zone Innovation Leading Talent Program (20143701)
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