Nucleosome Mobility and the Maintenance of Nucleosome Positioning
- 2 May 1997
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 276 (5313), 809-812
- https://doi.org/10.1126/science.276.5313.809
Abstract
To study nucleosome mobility and positioning, the R3 lac repressor was used with an adenosine triphosphate (ATP)–dependent chromatin assembly system to establish the positioning of five nucleosomes, with one nucleosome located between two R3 lac operators. When R3 protein was dissociated from DNA with isopropyl β- d -thiogalactopyranoside, the R3-induced nucleosome positions remained unchanged for at least 60 minutes in the absence of ATP but rearranged within 15 minutes in the presence of ATP. These results suggest that nucleosomes are dynamic and mobile rather than static and that a DNA binding factor is continuously required for the maintenance of nucleosome positioning.This publication has 37 references indexed in Scilit:
- Dimeric ligands define a role for transcriptional activation domains in reinitiationNature, 1996
- Chromatin: Pushing nucleosomes aroundCurrent Biology, 1996
- Interplay between chromatin structure and transcriptionCurrent Opinion in Cell Biology, 1995
- ATP-Dependent Nucleosome Reconfiguration and Transcriptional Activation from Preassembled Chromatin TemplatesScience, 1994
- Histones, nucleosomes and transcriptionCurrent Opinion in Genetics & Development, 1993
- Stable nucleosome positioning and complete repression by the yeast alpha 2 repressor are disrupted by amino-terminal mutations in histone H4.Journal of Bone and Joint Surgery, 1992
- A yeast protein that influences the chromatin structure of UASG and functions as a powerful auxiliary gene activator.Journal of Bone and Joint Surgery, 1990
- Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeastJournal of Molecular Biology, 1988
- The 5′ ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase INature, 1980
- Non-random cleavage of SV40 DNA in the compact minichromosome and free in solution by micrococcal nucleaseBiochemical and Biophysical Research Communications, 1980