A cationic fluorescent polymeric thermometer for the ratiometric sensing of intracellular temperature
Open Access
- 1 May 2015
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 140 (13), 4498-4506
- https://doi.org/10.1039/c5an00420a
Abstract
We developed new cationic fluorescent polymeric thermometers containing both benzothiadiazole and BODIPY units as an environment-sensitive fluorophore and as a reference fluorophore, respectively. The temperature-dependent fluorescence spectra of the thermometers enabled us to perform highly sensitive and practical ratiometric temperature sensing inside living mammalian cells. Intracellular temperatures of non-adherent MOLT-4 (human acute lymphoblastic leukaemia) and adherent HEK293T (human embryonic kidney) cells could be monitored with high temperature resolutions (0.01–1.0 °C) using the new cationic fluorescent polymeric thermometer.Keywords
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