Clinical grading of oral mucosa by curve‐fitting of corrected autofluorescence using diffuse reflectance spectra
- 13 October 2009
- journal article
- clinical trial
- Published by Wiley in Head & Neck
- Vol. 32 (6), 763-779
- https://doi.org/10.1002/hed.21251
Abstract
Background Laser‐induced autofluorescence (LIAF) and diffuse reflectance (DR) were collectively used in this clinical study to improve early oral cancer diagnosis and tissue grading. Methods LIAF and DR emission from oral mucosa were recorded on a fiber‐optic spectrometer by illumination with a 404‐nm diode laser and tungsten halogen lamp in 36 healthy volunteers and 40 lesions of 20 patients. Results Absorption dips in LIAF spectra at 545 and 575 nm resulting from changes in oxygenated hemoglobin were corrected using DR spectra of the same site. These corrected spectra were curve‐fitted using Gaussian spectral functions to determine constituent emission peaks and their relative contribution. The Gaussian peak intensity and area ratios F500/F635 and F500/F685 were found to be useful indicators of tissue transformation. The diagnostic capability of various ratios in differentiating healthy, hyperplastic, dysplastic, and squamous cell carcinomas (SCCs) were examined using discrimination scatterplots. Conclusions The LIAF/DR technique, in conjunction with curve‐fitting, differentiates different grades of dysplasia and SCC in this clinical trial and proves its potential for early detection of oral cavity cancer and tissue grading. © 2009 Wiley Periodicals, Inc. Head Neck, 2010Keywords
This publication has 47 references indexed in Scilit:
- Oxygenated hemoglobin diffuse reflectance ratio for in vivo detection of oral pre-cancerJournal of Biomedical Optics, 2008
- Probing local tissue changes in the oral cavity for early detection of cancer using oblique polarized reflectance spectroscopy: a pilot clinical trialJournal of Biomedical Optics, 2008
- In Vivo Brain Tumor Demarcation Using Optical Spectroscopy¶Photochemistry and Photobiology, 2007
- Optimal Excitation Wavelengths for In Vivo Detection of Oral Neoplasia Using Fluorescence Spectroscopy ¶Photochemistry and Photobiology, 2007
- Diagnosis of breast cancer using diffuse reflectance spectroscopy: Comparison of a Monte Carlo versus partial least squares analysis based feature extraction techniqueLasers in Surgery and Medicine, 2006
- Elastic scattering spectroscopy for the diagnosis of colonic lesions: initial results of a novel optical biopsy techniqueGastrointestinal Endoscopy, 2006
- Preliminary study of detecting neoplastic growths in vivo with real time calibrated autofluorescence imagingOptics Express, 2003
- Fluorescence spectroscopy of skinVibrational Spectroscopy, 2002
- Identification of Colonic Dysplasia and Neoplasia by Diffuse Reflectance Spectroscopy and Pattern Recognition TechniquesApplied Spectroscopy, 1998
- QUANTITATIVE OPTICAL SPECTROSCOPY FOR TISSUE DIAGNOSISAnnual Review of Physical Chemistry, 1996