hflB, a new Escherichia coli locus regulating lysogeny and the level of bacteriophage lambda cII protein
- 20 January 1986
- journal article
- Published by Elsevier BV in Journal of Molecular Biology
- Vol. 187 (2), 213-224
- https://doi.org/10.1016/0022-2836(86)90229-9
Abstract
The level of the viral cII protein has been proposed to be the crucial determinant in the lysis-lysogeny decision of bacteriophage lambda. A new Escherichia coli locus (hflB) has been identified in which a mutation (hflB29) leads to high frequency of lysogeny by λ. A double mutant defective in both hflB and the previously identified hflA gene displays a more severe Hfl− phenotype than either single mutant. The hflB locus is at 69 minutes on the E. coli map, 85% co-transducible with argG. The hflB29 mutation results in increased stability of the phage cII protein (increasing its half-life twofold) and is recessive to hflB+. We conclude that the hflB+ locus is a negative regulator of cII, perhaps coding for or regulating a protease that acts on cII. In addition, we observe that the can1 mutation, an alteration of the cII gene that results in enhanced lysogenization, leads to increased stability of cII protein. These observations reinforce the view that the level of cII is a key factor in the lysis-lysogeny decision of lambda.Keywords
This publication has 22 references indexed in Scilit:
- Bacteriophage λ protein cII binds promoters on the opposite face of the DNA helix from RNA polymeraseNature, 1983
- Control of phage λ development by stability and synthesis of cll protein: Role of the viral clll and host hflA, himA and himD genesCell, 1982
- Protein degradation in E. coli: The ion mutation and bacteriophage lambda N and cll protein stabilityCell, 1981
- THE LYSIS-LYSOGENY DECISION OF PHAGE λ: EXPLICIT PROGRAMMING AND RESPONSIVENESSAnnual Review of Genetics, 1980
- Structure and function of the cy control region of bacteriophage lambdaJournal of Molecular Biology, 1980
- The relationship between function and DNA sequence in an intercistronic regulatory region in phage λNature, 1978
- Genetic engineering in Vivo using translocatable drug-resistance elementsJournal of Molecular Biology, 1977
- Effect of mutations in the cII and cIII genes of bacteriophage λ on macromolecular synthesis in infected cellsJournal of Molecular Biology, 1970
- A site essential for expression of all late genes in bacteriophage λJournal of Molecular Biology, 1970
- Integration-negative mutants of bacteriophage lambdaJournal of Molecular Biology, 1968