Modulation of brain metabolism by very low concentrations of the commonly used drug delivery vehicle dimethyl sulfoxide (DMSO)
- 12 September 2007
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 86 (1), 208-214
- https://doi.org/10.1002/jnr.21477
Abstract
Dimethyl sulfoxide (DMSO) has long been used in studies as a vehicle to enhance the solubility and transport of ligands in biological systems. The effects of this drug on the outcomes of such studies are still unclear, with concentrations of DMSO reported as “safe” varying considerably. In the present work, we investigated the effects of very low concentrations of DMSO on the brain metabolism of [3-13C]pyruvate and D-[1-13C]glucose using 1H/13C NMR spectroscopy and a guinea pig cortical brain slice model. Our results show that DMSO is accumulated by brain slices. DMSO at all concentrations [0.000025%–0.25% (v/v)] increased the metabolic rate when [3-13C]pyruvate was used as a substrate and also in the presence of D-[1-13C]glucose (0.00025%–0.1% DMSO). These results are consistent with DMSO stimulating respiration, which it may do through altering the kinetics of ATP-requiring reactions. Our results also emphasize that there is no practical concentration of DMSO that can be used in metabolic experiments without effect. Therefore, care should be taken when evaluating the actions of drugs administered in combination with DMSO.Keywords
This publication has 37 references indexed in Scilit:
- Effectiveness of extracellular lactate/pyruvate for sustaining synaptic vesicle proton gradient generation and vesicular accumulation of GABAJournal of Neurochemistry, 2006
- Glucose is Necessary to Maintain Neurotransmitter Homeostasis during Synaptic Activity in Cultured Glutamatergic NeuronsJournal of Cerebral Blood Flow & Metabolism, 2006
- Role of AMPA Receptor Desensitization and the Side Effects of a DMSO Vehicle on Reticulospinal EPSPs and Locomotor ActivityJournal of Neurophysiology, 2005
- Regulation of mitochondrial gene expression by energy demand in neural cellsJournal of Neurochemistry, 2005
- Group I and II metabotropic glutamate receptors alter brain cortical metabolic and glutamate/glutamine cycle activity: a 13C NMR spectroscopy and metabolomic studyJournal of Neurochemistry, 2004
- Compartmentation of metabolism probed by [2-13C]alanine: improved 13C NMR sensitivity using a CryoProbe detects evidence of a glial metabolonNeurochemistry International, 2003
- Uncoupled ATP Hydrolysis and Thermogenic Activity of the Sarcoplasmic Reticulum Ca2+-ATPasePublished by Elsevier BV ,2002
- Effect of dimethyl sulfoxide on phosphoryl transfer catalyzed by yeast hexokinaseBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1992
- Dimethyl sulfoxide (DMSO) blocks GABA-induced current in rat dorsal root ganglion neuronsNeuroscience Letters, 1992
- Role of water activity on the rates of acetyl phosphate and ATP hydrolysisFEBS Letters, 1988