Non‐NMDA glutamate receptors modulate capsaicin induced c‐fos expression within trigeminal nucleus caudalis
- 1 June 1999
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 127 (3), 623-630
- https://doi.org/10.1038/sj.bjp.0702584
Abstract
1. We examined the effects of the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/kainate receptor antagonists 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and 1,2,3,4-tetrahydro-6-nitro-2,3-dioxo-benzol[f]quinoxaline-7-sulpho namide (NBQX), the kainate receptor antagonists gamma-(R-)-glutamylaminomethanesulphonic acid (GAMS) and 6,7,8,9-tetrahydro-5-nitro-1H-benz[g]indole-2,3-dione-3-oxime (NS-102), and the group III metabotropic glutamate receptor (mGluR) agonist 2-amino-4-phosphono-S-butanoic acid (L-AP4) on c-fos-like immunoreactivity (c-fos LI) in trigeminal caudalis (Sp5C), lateral reticular (LRt), medullary reticular (Md) and solitary tract (Sol) nuclei, after intracisternal injection of capsaicin in urethane anaesthetized Sprague-Dawley rats. 2. Few c-fos labelled cells were observed within Sp5C in capsaicin-vehicle treated animals. The number of positive c-fos cells increased by 17 fold after intracisternal capsaicin (5 nmol) administration. 3. Pretreatment with CNQX (0.02, 0.1, 0.6, 3 and 15 mg kg-1) or NBQX (0.01, 0.1 and 1 mg kg-1), administered intraperitoneally 15 min before capsaicin, significantly reduced labelled cells within Sp5C by a maximum of 45 and 34%, respectively. The number of c-fox LI cells within LRt, Md and Sol was not affected. Pretreatment with L-AP4 (1, 3 and 10 mg kg-1) decreased the number of Sp5C c-fos LI cells by a maximum of 30%, whereas GAMS (1 and 10 mg kg-1) and NS-102 (1 and 5 mg kg-1) did not show any significant effect. 4. These results suggest that blockade of AMPA receptors, but not kainate receptors, or the activation of group III mGluRs, decrease the response of Sp5C neurons to trigeminovascular activation. Thus, in addition to NMDA receptors, mGluRs and AMPA receptors may modulate cephalic pain and may provide a potential therapeutic target for antimigraine drugs.This publication has 75 references indexed in Scilit:
- The l-AP4 receptorGeneral Pharmacology: The Vascular System, 1997
- Pharmacology and Regional Distribution of the Binding of 6‐[3H]Nitro‐7‐Sulphamoylbenzo[f]‐Quinoxaline‐2,3‐Dione to Rat BrainJournal of Neurochemistry, 1996
- Excitatory amino release within spinal trigeminal nucleus after mustard oil injection into the temporomandibular joint region of the ratPain, 1996
- Electron microscopy of immunoreactivity patterns for glutamate and ?-aminobutyric acid in synaptic glomeruli of the feline spinal trigeminal nucleus (subnucleus caudalis)Journal of Comparative Neurology, 1996
- Selective block of recombinant glur6 receptors by NS-102, a novel non-NMDA receptor antagonistEuropean Journal of Pharmacology: Molecular Pharmacology, 1994
- Distinct gene expression of the N-methyl-d-aspartate receptor channel subunit in peripheral neurons of the mouse sensory ganglia and adrenal glandNeuroscience Letters, 1994
- The NMDA antagonist memantine blocks pain behavior in a rat model of formalin-induced facial painPain, 1993
- A novel non-NMDA receptor antagonist shows selective displacement of low-affinity [3H]kainate bindingEuropean Journal of Pharmacology: Molecular Pharmacology, 1993
- The effect of glutamate antagonists on c-fos expression induced in spinal neurons by irritation of the lower urinary tractBrain Research, 1992
- The NMDA receptor mediates cortical induction of fos and fos-related antigens following cortical injuryExperimental Neurology, 1990