NMR Spectroscopic Study of a DNA Duplex with Mercury-Mediated T-T Base Pairs
- 1 June 2006
- journal article
- research article
- Published by Taylor & Francis Ltd in Nucleosides, Nucleotides and Nucleic Acids
- Vol. 25 (4-6), 613-624
- https://doi.org/10.1080/15257770600686154
Abstract
Recently, we reported that T-T mismatches can specifically recognize Hg II (T-Hg II -T pair formation). In order to understand the properties of the T-Hg II -T pair, we recorded NMR spectra for a DNA duplex, d(CGCG TT GTCC) • d(GGAC TT CGCG), with two successive T-T mismatches (Hg II -binding sites). We assigned 1 H resonances for mercury-free and di-mercurated duplexes, and performed titration experiments with Hg II by using 1D 1 H NMR spectra. Because of the above mentioned assignments, we could confirm the existence of mono-mercurated species, because individual components gave independent NMR signals in the titration spectra.Keywords
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