Atmospheric pressure photo-ionization liquid chromatography/mass spectrometric determination of aflatoxins in food
- 1 January 2004
- journal article
- research article
- Published by Informa UK Limited in Food Additives & Contaminants
- Vol. 21 (1), 76-84
- https://doi.org/10.1080/02652030310001632538
Abstract
A comparison between electrospray ionization (ESI) and the recent introduced atmospheric pressure photo-ionization (APPI) technique is described for the liquid chromatography/mass spectrometric (LC/MS) determination of aflatoxin B1, B2, G1 and G2 in food. For the optimization of APPI, several APPI ion source parameters were examined. Furthermore, the chemical noise and signal suppression of ESI and APPI by sample matrix interference were investigated. The results indicated that APPI has the low chemical noise and the little signal suppression in comparison with ESI. The mean recovery of each aflatoxin from peanuts, corn, nutmeg and red pepper sample spiked at 1 ng g−1 ranged from 89 to 105% with relative standard deviations of 3.3–5.1%. The limit of detection (S/N = 3) in all food was in the range 0.11–0.5 ng g−1. From these studies, it is suggested that APPI can be used as an additional tool in food residue analysis.Keywords
This publication has 12 references indexed in Scilit:
- An application of liquid chromatography and mass spectrometry for determination of aflatoxinsJSM Mycotoxins, 2002
- Determination of aflatoxins B1, B2, G1 and G2 in spices using a multifunctional column clean-upJournal of Chromatography A, 2001
- Atmospheric Pressure Photoionization: An Ionization Method for Liquid Chromatography−Mass SpectrometryAnalytical Chemistry, 2000
- Determination of aflatoxins in food using LC/MS/MSZeitschrift Für Lebensmitteluntersuchung Und -Forschung A, 1998
- Determination of aflatoxins in peanut meal by LC/MS with a particle beam interfaceChromatographia, 1995
- Determination of aflatoxins in dust and urine by liquid chromatography/electrospray ionization tandem mass spectrometryRapid Communications in Mass Spectrometry, 1995
- Immunoaffinity Column Cleanup/Liquid Chromatographic Determination of Aflatoxins: An Interlaboratory StudyJournal of AOAC INTERNATIONAL, 1994
- Determination of aflatoxins by reversed-phase high-performance liquid chromatography with post-column in-line photochemical derivatization and fluorescence detectionJournal of Chromatography A, 1993
- The Use of HPLC/Thermospray Ms for the Confirmation of Aflatoxins in PeanutsJournal of Liquid Chromatography, 1991
- Reversed-phase high-performance liquid chromatographic analytical system for aflatoxins in wines with fluorescence detectionJournal of Chromatography A, 1977