Variation in the amount of petrolatum preparation applied at patch testing
- 14 December 2006
- journal article
- Published by Wiley in Contact Dermatitis
- Vol. 56 (1), 38-42
- https://doi.org/10.1111/j.1600-0536.2007.00985.x
Abstract
The elicitation of a positive patch test reaction in a given individual depends upon the dose of the sensitizer applied, the patch test technique and the occlusion time. The dose is determined by the concentration and volume/amount of test preparation applied. If the same amount/volume of a test preparation is applied all the time with the same test technique (same area of skin) and occlusion time, it is appropriate to use concentration as a dose parameter. Most contact sensitizers are incorporated in petrolatum (pet.). With pet. as vehicle, it is impossible to repeatedly apply an exact volume/amount. This study was performed to investigate the inter- and intra-individual variation of pet. preparation applied at patch testing by 3 technicians. Weighing demonstrated that the 3 technicians had about the same precision in their pet. application. The investigation demonstrates that there is both an inter-individual (statistically significant) and intra-individual variation in the amounts of pet. applied at patch testing for the 3 technicians. Presently, there is no recommendation on what amount of pet. preparation to apply, which merits a decision to be taken based on thorough investigations on the appropriate volumes of pet. preparation to be applied in various patch test systems.Keywords
This publication has 11 references indexed in Scilit:
- Recommendation to include methyldibromo glutaronitrile in the European standard patch test seriesContact Dermatitis, 2005
- Audit of Finn Chamber® patch test preparationContact Dermatitis, 2002
- Stability of corticosteroid patch test preparationsContact Dermatitis, 2000
- Thoughts on sensitizers in a standard patch test seriesContact Dermatitis, 1999
- Studies on the reproducibility of allergic contact dermatitisBritish Journal of Dermatology, 1996
- Studies on the reproducibility of allergic contact dermatitisBritish Journal of Dermatology, 1996
- High frequency of contact allergy to gold sodium thiosulfateContact Dermatitis, 1994
- Influence of area of application of allergen on sensitization in contact dermatitisContact Dermatitis, 1992
- Finn chamber patch test techniqueContact Dermatitis, 1984
- Studies on purity and stability of photopatch test substancesContact Dermatitis, 1983