Rabbit β-globin mRNA production in mouse L cells transformed with cloned rabbit β-globin chromosomal DNA
- 1 September 1979
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 281 (5726), 40-46
- https://doi.org/10.1038/281040a0
Abstract
Mouse thymidine kinase-negative L cells were transformed with a cloned rabbit chromosomal β-globin gene linked to the cloned thymidine kinase gene of herpes simplex virus type 1. Most thymidine kinase-positive cell lines contained one or more copies of rabbit β-globin DNA and produced up to 2,000 copies of rabbit β-globin RNA per cell indistinguishable from its authentic counterpart. No mouse β-globin mRNA was detected.Keywords
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