Gold Nanoparticle Based Surface-Enhanced Raman Scattering Spectroscopy of Cancerous and Normal Nasopharyngeal Tissues under Near-Infrared Laser Excitation
- 1 October 2009
- journal article
- Published by SAGE Publications in Applied Spectroscopy
- Vol. 63 (10), 1089-1094
- https://doi.org/10.1366/000370209789553291
Abstract
The capabilities of using gold nanoparticle based near-infrared surface-enhanced Raman scattering (SERS) to obtain biochemical information with high spatial resolution from human nasopharyngeal tissue were presented in this paper. The gold nanoparticles used have a mean diameter of 43 nm with a standard deviation of 6 nm. The SERS bands of nasopharyngeal tissue were assigned to known molecular vibrations of nucleic acids, amino acids, proteins, and metabolites. We also observed the blinking phenomenon at the tissue level when measuring the nasopharyngeal tissue SERS spectra, most frequently in signal intensity but also occasionally in peak positions. This phenomenon is excitation light intensity dependent. This work demonstrated great potential for using SERS imaging for distinguishing cancerous and normal nasopharyngeal tissues on frozen sections without using any dye labeling or other chemical species as functionalized binding sites.Keywords
This publication has 41 references indexed in Scilit:
- Ultrastable Substrates for Surface-Enhanced Raman Spectroscopy: Al2O3 Overlayers Fabricated by Atomic Layer Deposition Yield Improved Anthrax Biomarker DetectionJournal of the American Chemical Society, 2006
- Micro-Raman spectroscopic identification of bacterial cells of the genus Staphylococcus and dependence on their cultivation conditionsThe Analyst, 2005
- Characterization of the Surface Enhanced Raman Scattering (SERS) of BacteriaThe Journal of Physical Chemistry B, 2004
- Surface-Enhanced Raman Spectroscopy for Bacterial Discrimination Utilizing a Scanning Electron Microscope with a Raman Spectroscopy InterfaceAnalytical Chemistry, 2004
- Raman spectroscopy for optical diagnosis in normal and cancerous tissue of the nasopharynx—preliminary findingsLasers in Surgery and Medicine, 2003
- Nonresonant Raman imaging of protein distribution in single human cellsPeptide Science, 2002
- Nanoparticles with Raman Spectroscopic Fingerprints for DNA and RNA DetectionScience, 2002
- Raman Spectroscopy and Fluorescence Photon Migration for Breast Cancer Diagnosis and ImagingPhotochemistry and Photobiology, 1998
- Preparation and Characterization of Au Colloid MonolayersAnalytical Chemistry, 1995
- Surface-Enhanced Raman Gene ProbesAnalytical Chemistry, 1994