Effects of native and oxidized low density lipoproteins on formation and inactivation of endothelium-derived relaxing factor.
- 1 January 1991
- journal article
- abstracts
- Published by Ovid Technologies (Wolters Kluwer Health) in Arteriosclerosis and Thrombosis: A Journal of Vascular Biology
- Vol. 11 (1), 198-203
- https://doi.org/10.1161/01.atv.11.1.198
Abstract
The influence of native (N-) and oxidized (Ox-) low density lipoproteins (LDLs) on endothelium-dependent vasomotion is still controversial. We investigated the short-term effects of N-LDL and Ox-LDL on the formation of endothelium-derived relaxing factor (EDRF) in native and cultured endothelial cells and on its inactivation after release from the cells. N-LDL was isolated from fresh human plasma via sequential ultracentrifugation and oxidized by incubation with Cu2+. EDRF released from cultured endothelial cells was inactivated by both N-LDL and Ox-LDL (1 mg/ml) as detected in a bioassay system. N-LDL reduced the EDRF-mediated vasodilations of the detector segments by 38.5 +/- 5.3%, and Ox-LDL, by 55.5 +/- 4.6%. The effects of lipoproteins on EDRF formation were studied in cultured endothelial cells preincubated with either N-LDL or Ox-LDL (1 mg/ml for 1 hour) and stimulated for EDRF release with bradykinin after washout of the lipoproteins. EDRF was assessed by measuring its stimulatory effect on the activity of a purified, soluble guanylate cyclase. Both N-LDL and Ox-LDL did not reduce the bradykinin-induced EDRF formation. Consistent with this finding, acetylcholine-induced, EDRF-mediated dilations of intact rabbit femoral artery segments were not impaired by luminal exposure to N-LDL or Ox-LDL (1 mg/ml for 1 hour). However, these relaxations were significantly reduced by preincubation of aortic ring preparations with the same concentrations of the same charges of N-LDL and Ox-LDL. In conclusion, neither N-LDL nor Ox-LDL acutely impairs the formation of EDRF but does inactivate EDRF after its release from endothelial cells.(ABSTRACT TRUNCATED AT 250 WORDS)Keywords
This publication has 28 references indexed in Scilit:
- Beyond CholesterolNew England Journal of Medicine, 1989
- Enhanced responsiveness of smooth muscle, impaired endothelium-dependent relaxation and the genesis of coronary spasmThe American Journal of Cardiology, 1989
- Lipoproteins as factors in vessel tone and reactivity modulationBasic Research in Cardiology, 1988
- Isolation of low density lipoprotein from atherosclerotic vascular tissue of Watanabe heritable hyperlipidemic rabbits.Arteriosclerosis: An Official Journal of the American Heart Association, Inc., 1988
- Characterization of vascular relaxant factor released from cultured endothelial cells.Hypertension, 1987
- Impaired muscarinic endothelium-dependent relaxation and cyclic guanosine 5'-monophosphate formation in atherosclerotic human coronary artery and rabbit aorta.JCI Insight, 1987
- Lipoproteins Containing Apo B Extracted from Human Aortas Structure and FunctionaAnnals of the New York Academy of Sciences, 1985
- Endothelial cells are involved in the vasodilatory response to hypoxiaPflügers Archiv - European Journal of Physiology, 1983
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970