Enzyme-linked immuno-culture assay
- 1 March 1992
- journal article
- Published by Springer Science and Business Media LLC in Journal of Tissue Culture Methods
- Vol. 14 (1), 31-36
- https://doi.org/10.1007/bf01404545
Abstract
The current study outlines a procedure, designated enzyme-linked immuno-culture assay (ELICA), that will measure phenotypic expression of cultured cells in small plate assays. Given standard curves for phenotypic expression markers and in situ DNA analysis, this procedure will quantitate (to nanogram levels) intracellular, cell surface, or extracellular phenotypic expression markers; visualize the location of the markers; and determine DNA content, all within the same well of a 24- or 96-well tissue culture plate.Keywords
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