Single-particle cryo-EM-How did it get here and where will it go
Top Cited Papers
Open Access
- 31 August 2018
- journal article
- review article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 361 (6405), 876-+
- https://doi.org/10.1126/science.aat4346
Abstract
Cryo-electron microscopy, or simply cryo-EM, refers mainly to three very different yet closely related techniques: electron crystallography, single-particle cryo-EM, and electron cryotomography. In the past few years, single-particle cryo-EM in particular has triggered a revolution in structural biology and has become a newly dominant discipline. This Review examines the fascinating story of its start and evolution over the past 40-plus years, delves into how and why the recent technological advances have been so groundbreaking, and briefly considers where the technique may be headed in the future.Funding Information
- National Institutes of Health (R01GM082893)
- National Institutes of Health (R01GM098672)
- National Institutes of Health (R01HL134183)
- National Institutes of Health (P50GM082250)
- National Institutes of Health (P01GM111126)
- National Institutes of Health (S10OD020054)
- National Institutes of Health (S10OD021741)
- Howard Hughes Medical Institute
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