A rapid method for localized mutagenesis of yeast genes
- 1 February 1992
- Vol. 8 (2), 79-82
- https://doi.org/10.1002/yea.320080202
Abstract
We have developed a simple procedure for the localized mutagenesis of yeast genes. In this technique the region of interest is first amplified under mutagenic chain reaction (PCR) conditions. Cotransformation of the PCR product with a gapped plasmid containing homology to both ends the PCR procuct allows in vivo recombination to repair the gap with the mutagenized DNA. This procedure is efficient, allows trageting of specific regions for mutagenesis, and requires no subcloning steps in Escherichia coli.Keywords
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