Correlation of lymphocyte lipid composition membrane microviscosity and mitogen response in the aged
- 1 November 1991
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 21 (11), 2761-2765
- https://doi.org/10.1002/eji.1830211117
Abstract
Healthy aged and young blood donors were investigated for the role of membrane lipid composition in the age‐related increase in membrane microviscosity and decline of mitogen responsiveness. Membrane microviscosity was shown to correlate positively with membrane cholesterol/phospholipid molar ratios, which were significantly elevated in the elderly. A positive correlation also was confirmed between lymphocyte membrane microviscosity, which was measured using the probe 1,6‐diphenyl 1,3,5‐hexatriene, and phytohemagglutinin responsiveness of cells from the same donor. Using stepwise regression statistical analysis, the variables age, cholesterol, cholesterol/total phospholipid and phosphatidyl ethanolamine/phosphatidyl choline molar ratios were all shown to have a significant positive influence on membrane microviscosity, whereas total phospholipids had a negative effect. No statistically significant difference was seen in content of any single saturated or unsaturated fatty acid between young and old donors. After pooling, however, the proportion of all unsaturated fatty acids was significantly higher in cells from the elderly as a consequence of an increase of n‐6 and n‐3 polyunsaturated fatty acids. Changes in lipid composition and physical properties of lymphocyte plasma membranes may, therefore, be responsible (at least partially) for the diminution of immune reactivity in old age.Keywords
This publication has 13 references indexed in Scilit:
- Lipoprotein interactions with T cells: an updateImmunology Today, 1990
- Use of a FACS III for fluorescence depolarization with DPH.Journal of Histochemistry & Cytochemistry, 1989
- Approach to define “normal aging” in man. Immune function, serum lipids, lipoproteins and neopterin levelsMechanisms of Ageing and Development, 1988
- Analysis of fluorescent low density lipoprotein uptake by lymphocytes, paradoxical increase in the elderlyMechanisms of Ageing and Development, 1987
- Dietary fat alters progression of some age-related changes of the immune systemImmunology Letters, 1986
- Variations in dietary triacylglycerol saturation alter the lipid composition and fluidity of rat intestinal plasma membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1985
- Admission criteria for immunogerontological studies in man: The senieur protocolMechanisms of Ageing and Development, 1984
- Lipids and lymphocyte functionImmunology Today, 1984
- 273. Query: Multiple Comparison Methods for Comparing Regression CoefficientsBiometrics, 1970
- On a Test of Whether one of Two Random Variables is Stochastically Larger than the OtherThe Annals of Mathematical Statistics, 1947