Microarray-based analysis of cadmium-responsive microRNAs in rice (Oryza sativa)
Open Access
- 1 March 2011
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 62 (10), 3563-3573
- https://doi.org/10.1093/jxb/err046
Abstract
MicroRNAs (miRNAs) are a class of small non-coding RNAs that negatively regulate specific target mRNAs at the post-transcriptional level. Plant miRNAs have been implicated in developmental processes and adaptations to environmental stresses. Cadmium (Cd) is a non-essential heavy metal that is highly toxic to plants. To investigate the responsive functions of miRNAs under Cd stress, miRNA expression in Cd-stressed rice (Oryza sativa) was profiled using a microarray assay. A total of 19 Cd-responsive miRNAs were identified, of which six were further validated experimentally. Target genes were also predicted for these Cd-responsive miRNAs, which encoded transcription factors, and proteins associated with metabolic processes or stress responses. In addition, the mRNA levels of several targets were negatively correlated with the corresponding miRNAs under Cd stress. Promoter analysis showed that metal stress-responsive cis-elements tended to occur more frequently in the promoter regions of Cd-responsive miRNAs. These findings suggested that miRNAs played an important role in Cd tolerance in rice, and highlighted a novel molecular mechanism of heavy metal tolerance in plants.Keywords
This publication has 66 references indexed in Scilit:
- Cellular Response of Pea Plants to Cadmium Toxicity: Cross Talk between Reactive Oxygen Species, Nitric Oxide, and CalciumPlant Physiology, 2009
- Submergence-responsive MicroRNAs are Potentially Involved in the Regulation of Morphological and Metabolic Adaptations in Maize Root CellsAnnals of Botany, 2008
- Genome-wide analysis for discovery of rice microRNAs reveals natural antisense microRNAs (nat-miRNAs)Proceedings of the National Academy of Sciences of the United States of America, 2008
- Identification of novel and candidate miRNAs in rice by high throughput sequencingBMC Plant Biology, 2008
- Posttranscriptional Induction of Two Cu/Zn Superoxide Dismutase Genes in Arabidopsis Is Mediated by Downregulation of miR398 and Important for Oxidative Stress ToleranceTHE PLANT CELL ONLINE, 2006
- Characterization of a Novel Plant Promoter Specifically Induced by Heavy Metal and Identification of the Promoter Regions Conferring Heavy Metal ResponsivenessPlant Physiology, 2006
- AGO1 Homeostasis Entails Coexpression of MIR168 and AGO1 and Preferential Stabilization of miR168 by AGO1Molecular Cell, 2006
- Specific Effects of MicroRNAs on the Plant TranscriptomeDevelopmental Cell, 2005
- REACTIVE OXYGEN SPECIES: Metabolism, Oxidative Stress, and Signal TransductionAnnual Review of Plant Biology, 2004
- MicroRNAs in plantsGenes & Development, 2002