Caspases‐3 and ‐7 are activated in goniothalamin‐induced apoptosis in human Jurkat T‐cells
Open Access
- 11 August 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 456 (3), 379-383
- https://doi.org/10.1016/s0014-5793(99)00984-9
Abstract
Goniothalamin, a plant styrylpyrone derivative isolated from Goniothalamus andersonii, induced apoptosis in Jurkat T‐cells as assessed by the externalisation of phosphatidylserine. Immunoblotting showed processing of caspases‐3 and ‐7 with the appearance of their catalytically active large subunits of 17 and 19 kDa, respectively. Activation of these caspases was further evidenced by detection of poly(ADP‐ribose) polymerase cleavage (PARP). Pre‐treatment with the caspase inhibitor benzyloxycarbonyl‐Val‐Ala‐Asp fluoromethyl ketone (Z‐VAD.FMK) blocked apoptosis and the resultant cleavage of these caspases and PARP. Our results demonstrate that activation of at least two effector caspases is a key feature of goniothalamin‐induced apoptosis in Jurkat T‐cells.This publication has 27 references indexed in Scilit:
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