Pmr1, a Golgi Ca 2+ /Mn 2+ -ATPase, is a regulator of the target of rapamycin (TOR) signaling pathway in yeast
- 21 November 2006
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 103 (47), 17840-17845
- https://doi.org/10.1073/pnas.0604303103
Abstract
The rapamycin.FKBP12 complex inhibits target of rapamycin (TOR) kinase in TORC1. We screened the yeast nonessential gene deletion collection to identify mutants that conferred rapamycin resistance, and we identified PMR1, encoding the Golgi Ca2+/Mn2+ -ATPase. Deleting PMR1 in two genetic backgrounds confers rapamycin resistance. Epistasis analyses show that Pmr1 functions upstream from Npr1 and Gln-3 in opposition to Lst8, a regulator of TOR. Npr1 kinase is largely cytoplasmic, and a portion localizes to the Golgi where amino acid permeases are modified and sorted. Nuclear translocation of Gln-3 and Gln-3 reporter activity in pmr1 cells are impaired, but expression of functional Gap1 in the plasma membrane of a pmr1 strain in response to nitrogen limitation is enhanced. These two phenotypes suggest up-regulation of Npr1 function in the absence of Pmr1. Together, our results establish that Pmr1-dependent Ca2+ and/or Mn2+ ion homeostasis is necessary for TOR signaling.Keywords
This publication has 34 references indexed in Scilit:
- Transduction of the Nitrogen Signal Activating Gln3-mediated Transcription Is Independent of Npr1 Kinase and Rsp5-Bul1/2 Ubiquitin Ligase in Saccharomyces cerevisiaePublished by Elsevier BV ,2006
- Ammonia-specific Regulation of Gln3 Localization in Saccharomyces cerevisiae by Protein Kinase Npr1Published by Elsevier BV ,2006
- Multiple functions of the vacuolar sorting protein Ccz1p in Saccharomyces cerevisiaeBiochemical and Biophysical Research Communications, 2005
- TOR Complex 1 Includes a Novel Component, Tco89p (YPL180w), and Cooperates with Ssd1p to Maintain Cellular Integrity in Saccharomyces cerevisiaePublished by Elsevier BV ,2004
- Tor Kinases Are in Distinct Membrane-associated Protein Complexes inSaccharomyces cerevisiaeMolecular Biology of the Cell, 2003
- Role of Diacylglycerol in PKD Recruitment to the TGN and Protein Transport to the Plasma MembraneScience, 2002
- Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed)Nature Biotechnology, 2002
- The Npr1 Kinase Controls Biosynthetic and Endocytic Sorting of the Yeast Gap1 PermeasePublished by Elsevier BV ,2001
- HEAT Repeats Mediate Plasma Membrane Localization of Tor2p in YeastPublished by Elsevier BV ,2000
- Targets for Cell Cycle Arrest by the Immunosuppressant Rapamycin in YeastScience, 1991