Generation of Ppp2Ca and Ppp2Cb conditional null alleles in mouse
- 13 October 2011
- Vol. 50 (5), 429-436
- https://doi.org/10.1002/dvg.20815
Abstract
Protein phosphatase 2A (PP2A) is one of the most abundant serine/threonine phosphatases, with a critical role in embryonic development and human disease. There are two isoforms of the catalytic subunit of PP2A, Ppp2ca and Ppp2cb. Null mutation of Ppp2ca leads to early embryonic lethality at E6.5, hindering functional study of PP2A beyond this stage. We generated conditional null alleles of Ppp2ca and Ppp2cb by flanking with loxP sites exons 3 to 5 of Ppp2ca and exon 3 of Ppp2cb. Ppp2cafl/fl mice did not display any visible phenotype. Homozygous mutants in which Cre‐mediated excision resulted in global deletion of Ppp2ca displayed embryonic lethality and developmental defects similar to those previously reported. Ppp2cbΔ/Δ mice generated by the same strategy did not display any obvious morphological or physiological defects. These mouse strains can serve as important genetic tools to study the roles of PP2A during development and disease in a spatial‐ or temporal‐specific manner. genesis 50:429–436, 2012.Keywords
This publication has 17 references indexed in Scilit:
- Protein Phosphatase 2A Catalytic Subunit α (PP2Acα) Maintains Survival of Committed Erythroid Cells in Fetal Liver Erythropoiesis through the STAT5 PathwayThe American Journal of Pathology, 2011
- HnRNP U mediates the long-range regulation of Shh expression during limb developmentHuman Molecular Genetics, 2009
- Tid1, a Cochaperone of the Heat Shock 70 Protein and the Mammalian Counterpart of the Drosophila Tumor Suppressor l(2)tid, Is Critical for Early Embryonic Development and Cell SurvivalMolecular and Cellular Biology, 2004
- A Highly Efficient Recombineering-Based Method for Generating Conditional Knockout MutationsGenome Research, 2003
- Widespread recombinase expression using FLPeR (Flipper) micegenesis, 2000
- Type 2A protein phosphatase, the complex regulator of numerous signaling pathwaysBiochemical Pharmacology, 2000
- Protein phosphatase 2A is required for the initiation of chromosomal DNA replicationProceedings of the National Academy of Sciences of the United States of America, 1998
- Delayed embryonic lethality in mice lacking protein phosphatase 2A catalytic subunit CαProceedings of the National Academy of Sciences of the United States of America, 1998
- Constant expression and activity of protein phosphatase 2A in synchronized cells.Molecular and Cellular Biology, 1991
- Structure and transcriptional regulation of protein phosphatase 2A catalytic subunit genesBiochemistry, 1991