Anti-inflammatory protein TSG-6 reduces inflammatory damage to the cornea following chemical and mechanical injury
- 13 September 2010
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 107 (39), 16875-16880
- https://doi.org/10.1073/pnas.1012451107
Abstract
Previous reports demonstrated that adult stem/progenitor cells from bone marrow (multipotent mesenchymal stem cells; MSCs) can repair injured tissues with little evidence of engraftment or differentiation. In exploring this phenomenon, our group has recently discovered that the therapeutic benefits of MSCs are in part explained by the cells being activated by signals from injured tissues to express an anti-inflammatory protein TNF-α-stimulated gene/protein 6 (TSG-6). Therefore, we elected to test the hypothesis that TSG-6 would have therapeutic effects in inflammatory but noninfectious diseases of the corneal surface. We produced a chemical and mechanical injury of the cornea in rats by brief application of 100% ethanol followed by mechanical debridement of corneal and limbal epithelium. Recombinant human TSG-6 or PBS solution was then injected into the anterior chamber of the eye. TSG-6 markedly decreased corneal opacity, neovascularization, and neutrophil infiltration. The levels of proinflammatory cytokines, chemokines, and matrix metalloproteinases were also decreased. The data indicated that TSG-6, a therapeutic protein produced by MSCs in response to injury signals, can protect the corneal surface from the excessive inflammatory response following injury.This publication has 35 references indexed in Scilit:
- Ocular regeneration by stem cells: present status and future prospectsBritish Medical Bulletin, 2008
- Resolvin E1 and protectin D1 activate inflammation-resolution programmesNature, 2007
- Work-related eye injuries treated in hospital emergency departments in the USAmerican Journal of Industrial Medicine, 2005
- Inhibitory Effects of TSG‐6 Link Module on Leukocyte–Endothelial Cell Interactions In Vitro and In VivoMicrocirculation, 2004
- Cytokine-induced gene expression at the crossroads of innate immunity, inflammation and fertility: TSG-6 and PTX3/TSG-14Cytokine & Growth Factor Reviews, 2004
- The Link Module from Human TSG-6 Inhibits Neutrophil Migration in a Hyaluronan- and Inter-α-inhibitor-independent MannerPublished by Elsevier BV ,2002
- Reduced susceptibility to collagen‐induced arthritis in DBA/1J mice expressing the TSG‐6 transgeneArthritis & Rheumatism, 2002
- A Novel Allelic Variant of the Human TSG-6 Gene Encoding an Amino Acid Difference in the CUB ModulePublished by Elsevier BV ,2002
- Amelioration of collagen-induced arthritis in DBA/1J mice by recombinant TSG-6, a tumor necrosis factor/interleukin-1-inducible proteinArthritis & Rheumatism, 2000
- Thalidomide is an inhibitor of angiogenesis.Proceedings of the National Academy of Sciences of the United States of America, 1994