Whole‐Mount Histochemical Detection of β‐Galactosidase Activity
Open Access
- 1 April 2000
- journal article
- unit
- Published by Wiley in Current Protocols in Molecular Biology
- Vol. 50 (1), 14.14.1-14.14.8
- https://doi.org/10.1002/0471142727.mb1414s50
Abstract
This unit describes fixation and staining for b-galactosidase activity; it has been successfully used on vertebrate embryos and tissue explants. To help achieve consistent results with larger samples, an alternate protocol details preparation of thick sections. Support protocols are provided for clearing of tissues for better resolution and paraffin-embedding the whole embryo.Keywords
This publication has 14 references indexed in Scilit:
- The orphan nuclear receptor COUP-TFII is required for angiogenesis and heart developmentGenes & Development, 1999
- Molecular Distinction and Angiogenic Interaction between Embryonic Arteries and Veins Revealed by ephrin-B2 and Its Receptor Eph-B4Cell, 1998
- Male-specific cell migration into the developing gonadCurrent Biology, 1997
- Diabetes, defective pancreatic morphogenesis, and abnormal enteroendocrine differentiation in BETA2/NeuroD-deficient miceGenes & Development, 1997
- Cell autonomous functions of the receptor tyrosine kinase TIE in a late phase of angiogenic capillary growth and endothelial cell survival during murine developmentDevelopment, 1996
- Relationship between asymmetric nodal expression and the direction of embryonic turningNature, 1996
- A beta-galactosidase hybrid protein targeted to nuclei as a marker for developmental studies.Proceedings of the National Academy of Sciences of the United States of America, 1987
- Histochemical staining of clonal mammalian cell lines expressingE. coli ? galactosidase indicates heterogeneous expression of the bacterial geneSomatic Cell and Molecular Genetics, 1987
- New heat shock puffs and β=galactosidase activity resulting from transformation of Drosophila with an hsp70-lacZ hybrid geneCell, 1983
- Lac repressor can be fused to β-galactosidaseNature, 1974