Tissue Engineering for Total Meniscal Substitution: Animal Study in Sheep Model—Results at 12 Months
- 1 August 2012
- journal article
- research article
- Published by Mary Ann Liebert Inc in Tissue Engineering, Part A
- Vol. 18 (15-16), 1573-1582
- https://doi.org/10.1089/ten.tea.2011.0572
Abstract
The aim of the study was to investigate the use of a hyaluronic acid/polycaprolactone material for meniscal tissue engineering and to evaluate the tissue regeneration after the augmentation of the implant with expanded autologous chondrocytes. Eighteen skeletally mature sheep were treated. The animals were divided into three groups: cell-free scaffold, scaffold seeded with autologous chondrocytes, and meniscectomy alone. The implant was sutured to the capsule and to the meniscal ligament. At a 12-month gross assessment, histology and histomorphometry were used to assess the meniscus implant, knee joint, and osteoarthritis development. All implants showed excellent capsular ingrowth at the periphery. The implant gross assessment showed significant differences between cell-seeded and cell-free groups (p=0.011). The histological analysis indicated a cellular colonization throughout the implanted constructs. Avascular cartilaginous tissue formation was significantly more frequent in the cell-seeded constructs. Joint gross assessment showed that sheep treated with scaffold implantation achieved a significant higher score than those underwent meniscectomy (pp=0.047), even though results were not significantly superior to those of the cell-free scaffold. Seeding of the scaffold with autologous chondrocytes increases its tissue regeneration capacity, providing a better fibrocartilaginous tissue formation. The study suggests the potential of the novel hyaluronic acid/polycaprolactone scaffold for total meniscal substitution, although this approach has to be further improved before being applied into clinical practice.Keywords
This publication has 48 references indexed in Scilit:
- Biomechanical considerations in the pathogenesis of osteoarthritis of the kneeKnee Surgery, Sports Traumatology, Arthroscopy, 2011
- Stem Cell-Based Meniscus Tissue EngineeringTissue Engineering, Part A, 2011
- Engineered Cartilage Maturation Regulates Cytokine Production and Interleukin-1β ResponseClinical Orthopaedics & Related Research, 2011
- Tissue Ingrowth After Implantation of a Novel, Biodegradable Polyurethane Scaffold for Treatment of Partial Meniscal LesionsThe American Journal of Sports Medicine, 2011
- Changes in articular cartilage after meniscectomy and meniscus replacement using a biodegradable porous polymer implantKnee Surgery, Sports Traumatology, Arthroscopy, 2010
- Genipin enhances the mechanical properties of tissue‐engineered cartilage and protects against inflammatory degradation when used as a medium supplementJournal of Biomedical Materials Research Part A, 2008
- Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytesOsteoarthritis and Cartilage, 2007
- Meniscus Regeneration in a Rabbit Partial Meniscectomy ModelTissue Engineering, 1999
- Meniscal tissue regeneration in porous 50/50 copoly(l-lactide/ε-caprolactone) implantsBiomaterials, 1997
- Meniscal replacement using a porous polymer prosthesis: a preliminary study in the dogBiomaterials, 1996