Tumor rejection by local interferon gamma induction in established tumors is associated with blood vessel destruction and necrosis
Open Access
- 23 November 2010
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 128 (2), 371-378
- https://doi.org/10.1002/ijc.25350
Abstract
It has been shown that injecting a suspension of IFN-γ-secreting tumor cells results in their rejection. This effect has been attributed to IFN-γ preventing tumor stroma formation but not to a direct effect on the cancer cells. However, it is not known, which influence IFN-γ has on tumors with an established stroma. To address this question, the plasmacytoma cell line J558L was transduced with a vector allowing doxycycline-inducible IFN-γ gene expression. After the injection of the tumor cells into mice, IFN-γ was induced at different time points. Tumors did not grow when inducing IFN-γ immediately after tumor cell inoculation, while approximately half of the tumors were rejected when IFN-γ was induced in early established tumors within 2 weeks. Induction of IFN-γ 2–3 weeks after tumor cell inoculation was less efficient (0–17% rejection). IFN-γ induction in established tumors led to a reduction of CD146+ endothelial cells and massive necrosis. Together, we show that vascularized tumors can be rejected by local IFN-γ expression, but that rejection of established tumors was less efficient over time. This suggests that transplanted tumors became less susceptible to local IFN-γ treatment the better they are established.This publication has 26 references indexed in Scilit:
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