Construction of novel antibodies by use of DNA transfection: design of plasmid vectors
- 30 April 1986
- journal article
- Published by The Royal Society in Philosophical Transactions of the Royal Society of London. Series A, Mathematical and Physical Sciences
- Vol. 317 (1540), 425-432
- https://doi.org/10.1098/rsta.1986.0052
Abstract
The DNA that encodes antibodies can be manipulated in vitro and reintroduced into lymphoid cell lines. In this way, lymphocyte transfectants can be established which secrete milligram quantities of novel antibody molecules. Here we present data concerning the DNA sequences that are needed for efficient expression of the transfected antibody gene and give examples of the way in which this expression system for immunoglobulin gene DNA can be used for the production of useful antibody-related molecules.Keywords
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