Isolation, Structural Elucidation, Optical Resolution, and Antineuroinflammatory Activity of Phenanthrene and 9,10-Dihydrophenanthrene Derivatives from Bletilla striata
- 15 August 2019
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Natural Products
- Vol. 82 (8), 2238-2245
- https://doi.org/10.1021/acs.jnatprod.9b00291
Abstract
A phytochemical investigation of the aqueous EtOH extract of Bletilla striata tubers afforded 34 phenanthrene and 9,10-dihydrophenanthrene derivatives, including four new compounds, 1–4. These compounds were identified using physicochemical analyses and various spectroscopic methods. Twelve of these compounds were resolved into their enantiomers, and the absolute configurations were determined by comparison of experimental and calculated ECD spectra. The antineuroinflammatory activities were evaluated by measuring the inhibition of nitric oxide production in lipopolysaccharide-stimulated BV-2 microglial cells. Compounds 7, 32, and 33 displayed inhibitory activities, with IC50 values of 1.9, 5.0, and 1.0 μM, respectively, suggesting that they should be subjected to development as potential inhibitors of neuroinflammation.Keywords
Funding Information
- Ministry of Education of the People's Republic of China (N162004003)
- National Natural Science Foundation of China (81473108, 81473330, 81673323, 81872768, U1603125, 81628012)
- Liaoning Revitalization Talents Program (XLYC1807118)
- Program for Liaoning Excellent Talents in University (LR2015022)
- Liaoning BaiQianWan Talents Program
- State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University (CMEMR2017-B03)
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