Enhancing Nanoparticle-Based Visible Detection by Controlling the Extent of Aggregation
Open Access
- 13 June 2012
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 2 (1), 456
- https://doi.org/10.1038/srep00456
Abstract
Visible indication based on the aggregation of colloidal nanoparticles (NPs) is highly advantageous for rapid on-site detection of biological entities, which even untrained persons can perform without specialized instrumentation. However, since the extent of aggregation should exceed a certain minimum threshold to produce visible change, further applications of this conventional method have been hampered by insufficient sensitivity or certain limiting characteristics of the target. Here we report a signal amplification strategy to enhance visible detection by introducing switchable linkers (SLs), which are designed to lose their function to bridge NPs in the presence of target and control the extent of aggregation. By precisely designing the system, considering the quantitative relationship between the functionalized NPs and SLs, highly sensitive and quantitative visible detection is possible. We confirmed the ultrahigh sensitivity of this method by detecting the presence of 20 fM of streptavidin and fewer than 100 CFU/mL of Escherichia coli.Keywords
This publication has 28 references indexed in Scilit:
- Colorimetric Bacteria Sensing Using a Supramolecular Enzyme–Nanoparticle BiosensorJournal of the American Chemical Society, 2011
- Gold nanoparticle-based signal amplification for biosensingAnalytical Biochemistry, 2011
- Bioorthogonal chemistry amplifies nanoparticle binding and enhances the sensitivity of cell detectionNature Nanotechnology, 2010
- Gold Nanorod Based Selective Identification of Escherichia coli Bacteria Using Two-Photon Rayleigh Scattering SpectroscopyACS Nano, 2009
- Single chain fragment variable recombinant antibody functionalized gold nanoparticles for a highly sensitive colorimetric immunoassayBiosensors and Bioelectronics, 2009
- Gold Nanorod Probes for the Detection of Multiple PathogensSmall, 2008
- Rapid Detection of Escherichia coli by Using Antibody‐Conjugated Silver NanoshellsSmall, 2006
- Nanostructures in BiodiagnosticsChemical Reviews, 2005
- Controlled and Reversible Aggregation of Biotinylated Gold Nanoparticles with StreptavidinThe Journal of Physical Chemistry B, 2004
- Rapid Aggregation of Gold Nanoparticles Induced by Non-Cross-Linking DNA HybridizationJournal of the American Chemical Society, 2003