MicroRNA-518d regulates PPARα protein expression in the placentas of females with gestational diabetes mellitus
Open Access
- 17 March 2014
- journal article
- Published by Spandidos Publications in Molecular Medicine Reports
- Vol. 9 (6), 2085-2090
- https://doi.org/10.3892/mmr.2014.2058
Abstract
The placenta is thought to have a critical role in the pathogenesis of gestational diabetes mellitus (GDM), as GDM‑associated complications resolve following delivery. Placenta-specific microRNAs (miRNAs) may contribute to the pathology of the development of GDM. The aim of the present study was to evaluate whether the placenta-specific miR-518d contributes to the development of GDM. It was revealed that miR-518d expression was higher in placentas taken from patients with GDM compared with control placentas, whereas the protein levels of the predicted miR-518d target gene, peroxisome proliferator-activated receptor-α (PPARα), were lower in placentas from patients with GDM compared with those from control subjects. It was also demonstrated that PPARα was a direct target of miR-518d with a specific binding site at the seed sequence, which determines target specificity. In the placentas of females with GDM increased levels of miR-518d were negatively correlated with the levels of PPARα protein. As PPARα dysregulation may be related to the development of GDM, it is suggested that upregulation of miR-518d may be associated with the pathogenesis of GDM via an effect on the regulation of PPARα expression.Keywords
This publication has 20 references indexed in Scilit:
- MicroRNAs in Human Placental Development and Pregnancy ComplicationsInternational Journal of Molecular Sciences, 2013
- The Placenta and Gestational Diabetes MellitusCurrent Diabetes Reports, 2011
- Placental-specific microRNA in maternal circulation – identification of appropriate pregnancy-associated microRNAs with diagnostic potentialJournal of Reproductive Immunology, 2011
- Dysregulation of Leptin and Testosterone Production and Their Receptor Expression in the Human Placenta with Gestational Diabetes MellitusPlacenta, 2010
- First-Trimester Follistatin-Like-3 Levels in Pregnancies Complicated by Subsequent Gestational Diabetes MellitusDiabetes Care, 2009
- Human Villous Trophoblasts Express and Secrete Placenta-Specific MicroRNAs into Maternal Circulation via Exosomes1Biology of Reproduction, 2009
- Infants of Diabetic Mothers: The Effects of Hyperglycemia on the Fetus and NeonateNeonatal Network, 2007
- Increasing Prevalence of Gestational Diabetes MellitusDiabetes Care, 2007
- Gestational Diabetes Mellitus: Clinical Predictors and Long-Term Risk of Developing Type 2 DiabetesDiabetes Care, 2007
- Endocrine Regulation of Human Fetal Growth: The Role of the Mother, Placenta, and FetusEndocrine Reviews, 2006