The HSA Domain of BRG1 Mediates Critical Interactions Required for Glucocorticoid Receptor-Dependent Transcriptional Activation In Vivo
- 1 February 2008
- journal article
- Published by Informa UK Limited in Molecular and Cellular Biology
- Vol. 28 (4), 1413-1426
- https://doi.org/10.1128/mcb.01301-07
Abstract
The packaging of eukaryotic DNA into chromatin can create an impediment to transcription by hindering binding of essential factors required for transcription. The mammalian SWI/SNF remodeling complex has been shown to alter local chromatin structure and facilitate recruitment of transcription factors. BRG1 (or hBrm), the central ATPase of the human SWI/SNF complex, is a critical factor for the functional activity of nuclear receptor complexes. Analysis using BRG1/SNF2h chimeras suggests BRG1 may contain previously uncharacterized functional motifs important for SWI/SNF. To identify these regions, BRG1 truncation and deletion mutants were designed, characterized, and utilized in a series of assays to evaluate transcriptional activation and chromatin remodeling by the glucocorticoid receptor. We identified a domain within the N terminus of BRG1 that mediates critical protein interactions within SWI/SNF. We find the HSA domain of BRG1 is required to mediate the interaction with BAF250a/ARID1A and show this association is necessary for transcriptional activation from chromatin mouse mammary tumor virus or endogenous promoters in vivo. These studies suggest BAF250a is a necessary facilitator of BRG1-mediated chromatin remodeling required for SWI/SNF-dependent transcriptional activation.Keywords
This publication has 40 references indexed in Scilit:
- Nuclear receptors and chromatin remodeling machineryMolecular and Cellular Endocrinology, 2007
- Chromatin Modifications and Their FunctionCell, 2007
- Chromatin remodelling: the industrial revolution of DNA around histonesNature Reviews Molecular Cell Biology, 2006
- A Brg1 mutation that uncouples ATPase activity from chromatin remodeling reveals an essential role for SWI/SNF-related complexes in β-globin expression and erythroid developmentGenes & Development, 2005
- PBAF chromatin-remodeling complex requires a novel specificity subunit, BAF200, to regulate expression of selective interferon-responsive genesGenes & Development, 2005
- Reconstitution of Glucocorticoid Receptor-Dependent Transcription In VivoMolecular and Cellular Biology, 2004
- BAF60a Mediates Critical Interactions between Nuclear Receptors and the BRG1 Chromatin-Remodeling Complex for TransactivationMolecular and Cellular Biology, 2003
- Largest Subunits of the Human SWI/SNF Chromatin-remodeling Complex Promote Transcriptional Activation by Steroid Hormone ReceptorsJournal of Biological Chemistry, 2002
- Chromatin-remodelling factor BRG1 selectively activates a subset of interferon-α-inducible genesNature, 2002
- Systematic Identification of Novel Protein Domain Families Associated with Nuclear FunctionsGenome Research, 2002