Surface-Enhanced Raman Scattering-Based Sensing In Vitro: Facile and Label-Free Detection of Apoptotic Cells at the Single-Cell Level
- 18 February 2013
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 85 (5), 2809-2816
- https://doi.org/10.1021/ac303337b
Abstract
Surface-enhanced Raman scattering (SERS) is well recognized as a powerful analytical tool, enabling ultrahigh sensitive detection of analytes at low concentrations, even down to single-molecule level. Of particular note, in comparison to sufficient investigations on SERS-based detection of biomolecules (e.g., DNA and protein), there has been relatively scanty information regarding in vitro and in vivo detection. In this Article, we demonstrate a kind of SERS-active substrate, i.e., AgNPs-decorated silicon wafer (AgNPs@Si), as a high-performance in vitro sensing platform for single-cell detection of apoptotic cells. The AgNPs@Si yields highly reproducible SERS signals with an enhancement factor of ∼10(7). Remarkably, cellular experiments show that facile, noninvasive, label-free, and sensitive detection of apoptotic cells is readily realized using the high-performance SERS-active platform. Three kinds of apoptotic cells treated with apoptosis inducer are facilely and sensitively detected at the single-cell level, suggesting the exciting potential of AgNPs@Si for SERS-based in vitro analysis and detection.Keywords
This publication has 58 references indexed in Scilit:
- Noble Metal Coated Single-Walled Carbon Nanotubes for Applications in Surface Enhanced Raman Scattering Imaging and Photothermal TherapyJournal of the American Chemical Society, 2012
- Gold Nanoparticles-Decorated Silicon Nanowires as Highly Efficient Near-Infrared Hyperthermia Agents for Cancer Cells DestructionNano Letters, 2012
- Regiospecific Plasmonic Assemblies for in Situ Raman Spectroscopy in Live CellsJournal of the American Chemical Society, 2011
- Silver nanosheet-coated inverse opal film as a highly active and uniform SERS substrateJournal of Materials Chemistry, 2011
- In Situ Intracellular Spectroscopy with Surface Enhanced Raman Spectroscopy (SERS)-Enabled NanopipettesACS Nano, 2009
- Multiplexed imaging of surface enhanced Raman scattering nanotags in living mice using noninvasive Raman spectroscopyProceedings of the National Academy of Sciences of the United States of America, 2009
- Label-Free Separation of Human Embryonic Stem Cells and Their Cardiac Derivatives Using Raman SpectroscopyAnalytical Chemistry, 2009
- Dimers of Silver Nanospheres: Facile Synthesis and Their Use as Hot Spots for Surface-Enhanced Raman ScatteringNano Letters, 2008
- Aptamer-Based SERRS Sensor for Thrombin DetectionNano Letters, 2008
- Reductive Self-assembling of Pd and Rh Nanoparticles on Silicon Nanowire TemplatesChemistry of Materials, 2004